PMID: 11336223May 5, 2001Paper

Expressed sequence tags for the chicken genome from a normalized 10-day-old White Leghorn whole embryo cDNA library: 1. DNA sequence characterization and linkage analysis

The Journal of Heredity
E J SmithJ Foster

Abstract

Expressed sequence tags (ESTs) provide a rapid and reliable method for gene discovery as well as a resource for the large-scale analysis of gene expression of known and unknown genes. Here we describe a normalized cDNA library developed from a 10-day-old White Leghorn chicken whole embryo. The utility of the library was evaluated by partial sequencing of 99 randomly selected insert-containing clones and the analysis of EST-targeted genomic regions for single nucleotide polymorphisms (SNPs) in the East Lansing chicken reference DNA mapping panel. Using stringent match criteria of percent identity of 80 or higher across a length of 50 or more bases, 46 ESTs matched database sequences including previously reported Gallus gallus genes. Thirty-seven of the 50 primer pairs developed from 50 unique ESTs amplified a single fragment. The size of the 37 amplicons ranged from 276 to 693 bp for a total of 17,508 and an average of 473. About 70% of the SNPs detected were either G-->A or C-->T transition. The number of SNPs detected within the amplicons from EST-targeted genomic regions ranged from 0 to 4 for a total of 65 and a frequency of about 1 every 470 bases. About 35% of the amplicons contained only 1 SNP, while 19% had 4 SNPs. Using...Continue Reading

Citations

Jul 17, 2008·Comparative and Functional Genomics·Stefanie HartmanEd Smith
Apr 30, 2005·Biochemical Genetics·Edward J SmithRobert Gogal
May 21, 2005·Cytogenetic and Genome Research·M SchmidS Mizuno
Sep 18, 2004·Animal Genetics·C J FitzsimmonsL Andersson
Jan 9, 2003·Animal Biotechnology·K M Reed
May 29, 2004·Genetics·Hannah SundströmHans Ellegren
Nov 8, 2007·The Journal of Heredity·Xiaojing GuanEdward J Smith
Oct 26, 2011·Reproduction in Domestic Animals = Zuchthygiene·Mu-Tzu ChangMu-Chiou Huang

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