Expressed sequence tags from a NaCl-treated Suaeda salsa cDNA library

Gene
L ZhangHui Zhang

Abstract

Past efforts to improve plant tolerance to osmotic stress have had limited success owing to the genetic complexity of stress responses. The first step towards cataloging and categorizing genetically complex abotic stress responses is the rapid discovery of genes by the large-scale partial sequencing of randomly selected cDNA clones or expressed sequence tags (ESTs). Suaeda salsa, which can survive seawater-level salinity, is a favorite halophytic model for salt tolerant research. We constructed a NaCl-treated cDNA library of Suaeda salsa and sequenced 1048 randomly selected clones, out of which 1016 clones produced readable sequences (773 showed homology to previously identified genes, 227 matched unknown protein coding regions, 16 anomalous sequences or sequences of bacterial origin were excluded from further analysis). By sequence analysis we identified 492 unique clones: 315 showed homology to previously identified genes, 177 matched unknown protein coding regions (101 of which have been found before in other organisms and 76 are completely novel). All our EST data are available on the Internet. We believe that our dbEST and the associated DNA materials will be a useful source to scientists engaging in stress-tolerance study.

References

Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
Jan 8, 1999·Plant Physiology·D E NelsonH J Bohnert
Mar 6, 1999·Current Opinion in Plant Biology·H J Bohnert, E Sheveleva
May 14, 1999·Current Opinion in Plant Biology·M L NuccioA D Hanson
Mar 14, 2000·Current Opinion in Plant Biology·J C Cushman, H J Bohnert
Apr 5, 2000·The Journal of Biological Chemistry·F PosasJ Ariño
Apr 5, 2000·Biochimica Et Biophysica Acta·E BlumwaldM P Apse
Jun 6, 2000·Current Opinion in Plant Biology·J Ohlrogge, C Benning
Nov 18, 2000·Plant Physiology·J K Zhu
Jul 1, 1995·The Plant Cell·H. J. BohnertR. G. Jensen

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Citations

Aug 30, 2011·Plant Cell Reports·Selvam AyarpadikannanJai-Heon Lee
Feb 29, 2008·Functional & Integrative Genomics·Niranjan BaisakhPritish Varadwaj
Sep 2, 2003·Fungal Genetics and Biology : FG & B·Nuno T Sacadura, Barry J Saville
Jul 8, 2011·Journal of Proteome Research·Juanjuan YuShaojun Dai
Oct 25, 2011·Journal of Proteome Research·Heng ZhangShaojun Dai
Jun 27, 2013·Journal of Proteome Research·Le YangHaiying Li
Dec 18, 2013·Analytical and Bioanalytical Chemistry·Anna Laura CapriottiAldo Laganà
Apr 5, 2007·Journal of Genetics·M N JitheshAjay K Parida
Sep 6, 2012·Ecotoxicology and Environmental Safety·Huifeng WuJunbao Yu
Feb 15, 2003·FEBS Letters·Min Kyoung YouJung Myung Bae
Nov 20, 2008·Journal of Integrative Plant Biology·Yiyue ZhangQi Xie
Mar 17, 2007·DNA Sequence : the Journal of DNA Sequencing and Mapping·Shi-Hua ChenHui Zhang
Nov 10, 2004·Genomics·B Altenberg, K O Greulich
Dec 1, 2005·Proteomics·Brigitte AltenbergKarl Otto Greulich
Jun 10, 2011·Molecular Biology Reports·Narendra Singh YadavBhavanath Jha

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