Expression Analyses of Embryogenesis-Associated Genes during Somatic Embryogenesis of Adiantum capillus-veneris L. In vitro : New Insights into the Evolution of Reproductive Organs in Land Plants

Frontiers in Plant Science
Xia LiGuang-Yuan Rao

Abstract

An efficient in vitro regeneration system via somatic embryogenesis (SE) was developed for a fern species Adiantum capillus-veneris. Adventitious shoots, green globular bodies (GGBs) and calli were obtained with the maximal induction rate on the Murashige and Skoog (MS) medium of low concentrations of 6-benzyladenine (BA) (0-1.0 mg/L), 2.0 mg/L BA without 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5 mg/L 2,4-D and 0.5-1.0 mg/L 6-BA, respectively. Cyto-morphological and histological changes in the shoot development via calli and GGBs were examined. For a better understanding of these developmental events, expression patterns of six genes, AcLBD16, AcAGL, AcBBM, AcWUS, AcRKD, and AcLEC1, were characterized during SE. AcBBM and AcLEC1 were ubiquitously expressed in direct SE (adventitious shoots and GGBs) the maximal expression of AcBBM in mature GGBs, and the high expression of AcLEC1 in GGB initiation and adventitious shoots. During the indirect SE, AcLBD16, AcLEC1, AcRKD, and AcWUS were highly expressed in mature calli. Additionally, phylogenetic analyses showed that AcWUS, AcBBM, AcLBD, AcAGL, AcRKD, and their homologs of other green plants formed monophyletic clades, respectively. Some of these gene families, however, diversif...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 10, 2002·The Plant Journal : for Cell and Molecular Biology·Jianru ZuoNam-Hai Chua
Aug 13, 2002·The Plant Cell·Kim BoutilierMichiel M van Lookeren Campagne
Feb 18, 2004·Journal of Experimental Botany·Claudio StasollaRonald R Sederoff
Jul 22, 2004·Plant Physiology·Iris TzafrirDavid Meinke
Jan 14, 2005·Bioinformatics·Federico AbascalDavid Posada
Aug 4, 2005·Development·Pablo D Jenik, M Kathryn Barton
Jan 26, 2007·Journal of Plant Research·Masamitsu Wada
Oct 28, 1994·Science·R B GoldbergR Yadegari
Oct 15, 2008·Systematic Biology·Alexandros StamatakisJacques Rougemont
Mar 11, 2009·Annals of Botany·Burkhard Becker, Birger Marin
Aug 28, 2010·Plant Molecular Biology·Souad El OuakfaouiBrian Miki
Mar 15, 2011·Current Biology : CB·Akira IwaseMasaru Ohme-Takagi
Apr 5, 2011·The Plant Journal : for Cell and Molecular Biology·Dávid KoszegiHelmut Bäumlein
Jan 11, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Nuno D Pires, Liam Dolan
Jan 19, 2013·Molecular Biology and Evolution·Kazutaka Katoh, Daron M Standley
Sep 13, 2013·Frontiers in Plant Science·Alejandra VascoBarbara A Ambrose
Oct 1, 2013·The Plant Cell·Momoko IkeuchiAkira Iwase
Feb 11, 2014·TheScientificWorldJournal·Gaibin LianJie Xu
Jul 16, 2014·Trends in Plant Science·Tatyana Radoeva, Dolf Weijers
Jul 20, 2014·Biochimica Et Biophysica Acta·Attila Fehér
Apr 4, 2015·Molecular Biology and Evolution·Andre S ChanderbaliDouglas E Soltis
Jan 1, 2015·Nature Plants·Momoko IkeuchiKeiko Sugimoto
Jun 28, 2016·Current Biology : CB·Satoshi KoiKeiji Nakajima
Jul 26, 2016·Frontiers in Plant Science·Yuan GuanZhen-Hong Su

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Methods Mentioned

BETA
scanning electron microscopy
RNA-Seq

Software Mentioned

Protest
RaxML
euANT
Axioplan
SPSS
MAFFT

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