Expression analysis of Arabidopsis thaliana NAD-dependent isocitrate dehydrogenase genes shows the presence of a functional subunit that is mainly expressed in the pollen and absent from vegetative organs

Plant & Cell Physiology
Thomas Lemaitre, Michael Hodges

Abstract

NAD-dependent isocitrate dehydrogenase (IDH) is a Krebs cycle enzyme situated in mitochondria. In Arabidopsis thaliana, five genes encode functional IDH subunits that can be classed into two groups based on gene structure and subunit amino acid sequence. Arabidopsis contains two 'catalytic' and three 'regulatory' subunits according to their homology with yeast IDH. To date, an active IDH is believed to be heteromeric, containing at least one of each subunit type. This was verified in Arabidopsis by the complementation of yeast IDH mutants with the different Arabidopsis IDH-encoding cDNAs. Indeed, a single 'catalytic' and 'regulatory' subunit was sufficient to restore acetate growth of the yeast IDH double mutant. To gain information on possible IDH subunit interactions in planta, Arabidopsis IDH gene expression was analysed by Northern blot, PCR on cDNA libraries, in silico and in 'promoter'-reporter gene transgenic plants. Four of the IDH genes were expressed in all plant organs tested, while one gene (At4g35650) was not expressed in vegetative organs but was mainly expressed in the pollen. In leaves, the IDH genes were highly expressed in the veins, and to a lesser extent in mesophyll cells. The data are discussed with respec...Continue Reading

References

Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Oct 24, 2000·The Journal of Biological Chemistry·E A Panisko, L McAlister-Henn
Sep 15, 2001·Free Radical Biology & Medicine·K I Minard, L McAlister-Henn
Apr 16, 2002·The Journal of Biological Chemistry·An-Ping Lin, Lee McAlister-Henn
Feb 4, 2003·The Journal of Biological Chemistry·An-Ping Lin, Lee McAlister-Henn
Jun 14, 2003·Plant Physiology·David Honys, David Twell
Sep 18, 2004·Plant Physiology·Philip ZimmermannWilhelm Gruissem
Nov 13, 2004·Genome Biology·David Honys, David Twell
Apr 5, 2005·Nature Genetics·Markus SchmidJan U Lohmann

❮ Previous
Next ❯

Citations

Feb 6, 2010·The Journal of Biological Chemistry·Marcos A TronconiMaría F Drincovich
Nov 15, 2012·BMC Research Notes·Jullyana Cristina Magalhães Silva MouraPaulo Mazzafera
Feb 3, 2007·The Plant Journal : for Cell and Molecular Biology·Shin-nosuke HashidaHirofumi Uchimiya
May 31, 2013·The New Phytologist·Edouard R A Boex-FontvieilleGuillaume G B Tcherkez
May 9, 2014·Frontiers in Plant Science·Peter Schertl, Hans-Peter Braun
May 17, 2017·Nature Communications·Youjun ZhangToshihiro Obata
Apr 4, 2008·Molecular & Cellular Proteomics : MCP·Chun Pong LeeA Harvey Millar
Jan 11, 2019·Antioxidants·Adnan Khan NiaziJean-Philippe Reichheld
Mar 1, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wang-Gang TangGuo-Ping Zhu
Jan 1, 2008·The Arabidopsis Book·A Harvey MillarJames Whelan
Dec 24, 2020·Plant & Cell Physiology·Sakuya NakamuraMasanori Izumi
May 10, 2021·Biochimica Et Biophysica Acta. Bioenergetics·Nils RugenHolger Eubel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.