Expression Analysis of Genes Involved in the RB/E2F Pathway in Astrocytic Tumors

PloS One
Wallax Augusto Silva FerreiraBárbara do Nascimento Borges

Abstract

Astrocytic gliomas, which are derived from glial cells, are considered the most common primary neoplasias of the central nervous system (CNS) and are histologically classified as low grade (I and II) or high grade (III and IV). Recent studies have shown that astrocytoma formation is the result of the deregulation of several pathways, including the RB/E2F pathway, which is commonly deregulated in various human cancers via genetic or epigenetic mechanisms. On the basis of the assumption that the study of the mechanisms controlling the INK4/ARF locus can help elucidate the molecular pathogenesis of astrocytic tumors, identify diagnostic and prognostic markers, and help select appropriate clinical treatments, the present study aimed to evaluate and compare methylation patterns using bisulfite sequencing PCR and evaluate the gene expression profile using real-time PCR in the genes CDKN2A, CDKN2B, CDC6, Bmi-1, CCND1, and RB1 in astrocytic tumors. Our results indicate that all the evaluated genes are not methylated independent of the tumor grade. However, the real-time PCR results indicate that these genes undergo progressive deregulation as a function of the tumor grade. In addition, the genes CDKN2A, CDKN2B, and RB1 were underexpres...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·J G HermanS B Baylin
Oct 6, 1997·Cancer Genetics and Cytogenetics·N Ohtani-FujitaT Sakai
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z YanR S Williams
Oct 17, 1998·Molecular and Cellular Biology·G HateboerK Helin
Mar 31, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M JönssonT Andersson
Mar 21, 2001·Human Molecular Genetics·J R Nevins
Feb 2, 2002·Nature Reviews. Molecular Cell Biology·Jeffrey M Trimarchi, Jacqueline A Lees
Jul 11, 2002·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·L NakopoulouP S Davaris
Jul 16, 2002·Carcinogenesis·Esteban Ballestar, Manel Esteller
Aug 9, 2002·Molecular and Cellular Biology·Stuart S LevineRobert E Kingston
Nov 15, 2002·Cancer Biology & Therapy·J Alan Diehl
Apr 18, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Yukitaka UshioHideyuki Saya
May 3, 2003·Cancer Cell·Hugh Cam, Brian David Dynlacht
Jun 27, 2003·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P Gonzalez-GomezJ A Rey
Jan 15, 2004·The Journal of Clinical Investigation·In-Kyung ParkMichael F Clarke
Oct 7, 2004·Cancer Research·Hiroko OhgakiPaul Kleihues
Nov 17, 2004·Genes & Development·Charles J Sherr, James M Roberts
Nov 26, 2004·The Journal of Biological Chemistry·Jan Willem VonckenUlf R Rapp
Jan 22, 2005·Nature Reviews. Cancer·Michael A GonzalezNicholas Coleman
Mar 11, 2005·The New England Journal of Medicine·Roger StuppUNKNOWN National Cancer Institute of Canada Clinical Trials Group
Apr 20, 2005·Oncogene·Amit DeshpandePhilip W Hinds
Jun 30, 2005·Cancer Metastasis Reviews·Ruth Halaban
Jan 28, 2006·EMBO Reports·Eric LauWei Jiang
Jul 21, 2006·Cell Cycle·Susana Gonzalez, Manuel Serrano

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Citations

Apr 20, 2018·Pharmacological Reviews·Andrea ShergalisNouri Neamati
Feb 6, 2020·Anti-cancer Agents in Medicinal Chemistry·Wallax A S FerreiraEdivaldo H Correa de Oliveira
Mar 10, 2020·World Journal of Gastroenterology : WJG·Han-Zhang ZhuChang-Ku Jia
May 7, 2020·Bioscience Reports·Anum Zehra NaqviMahmood Akhtar Kayani
Oct 14, 2020·Cell Biology International·S Daisy PrecillaAnitha Thirugnanasambandhar Sivasubramanian
Jan 21, 2021·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·W A S FerreiraE H C de Oliveira

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Methods Mentioned

BETA
surgical resection
biopsies
electrophoresis
PCR
deamination
acetylation

Software Mentioned

BISMA ( Bisulfite Sequence DNA Methylation Analysis )
BiQ Analyzer
GraphPad Prism 5
BioEdit

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