Expression analysis of GSK-3β in diapause pupal brains in the cotton bollworm, Helicoverpa armigera

Insect Science
Wei Chen, Wei-Hua Xu

Abstract

Diapause is an adaptive response to adverse environmental conditions, but the molecular mechanisms are unclear. Some signaling molecules have been identified in the regulation of diapause. GSK-3β is an important signaling protein involved in several signaling pathways. In this study, GSK-3β from the cotton bollworm, Helicoverpa armigera, was cloned using reverse transcription polymerase chain reaction and rapid amplification of complementary DNA (cDNA) ends techniques. Sequence analysis showed that the full-length cDNA was 1447 bp containing a 292 bp 5'-untranslated region (UTR), a 162 bp 3'-UTR and a 993 bp open reading frame (ORF). The deduced Har-GSK-3β protein has high identity to other known GSK-3β, as determined by Basic Local Alignment Search Tool analysis. Developmental expression of total GSK-3β and p-GSK-3β (Ser9) in diapause and non-diapause pupal brains was investigated by Western blotting. Results indicated that the activity of GSK-3β is down-regulated in diapause pupal brains, which is further confirmed by Western blotting after diapause break. These finding suggest that the down-regulation of Har-GSK-3β activity may be important for pupal diapause.

References

Sep 21, 2001·The Biochemical Journal·S Frame, P Cohen
Oct 2, 2001·Science's STKE : Signal Transduction Knowledge Environment·J R Woodgett
Dec 1, 2001·Annual Review of Entomology·David L Denlinger
Dec 20, 2005·Insect Biochemistry and Molecular Biology·Yoshihiro FujiwaraKunihiro Shiomi
Oct 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Karen D WilliamsMarla B Sokolowski
Oct 24, 2006·Nature Reviews. Molecular Cell Biology·Philip Cohen
Nov 10, 2007·The Journal of Biological Chemistry·David J PankaJames W Mier
Feb 6, 2008·The Journal of Biological Chemistry·Diana HoogeboomBoudewijn M Th Burgering
May 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Cheolho Sim, David L Denlinger
Oct 24, 2009·The Journal of Biological Chemistry·Ser Sue NgHans Clevers
Aug 10, 2010·Annual Review of Entomology·Daniel A Hahn, David L Denlinger
Aug 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Wei-Hua XuDavid L Denlinger
Jul 20, 2014·Insect Biochemistry and Molecular Biology·Wei Chen, Wei-Hua Xu

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Citations

Dec 17, 2020·Frontiers in Physiology·Yan-Juan DingCan Li

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