Expression analysis of the NgORF13 promoter during the development of tobacco genetic tumors

Plant & Cell Physiology
Makiko UdagawaK Syöno

Abstract

We investigated the expression pattern of the promoter of Nicotiana glauca (Ng) ORF13 in the hybrids between N. glauca and N. langsdorffii harboring the NgORF13-beta-glucuronidase (GUS) chimeric gene. The promoter of NgORF13 of N. glauca had lower activities than the promoter of RiORF13 of Agrobacterium rhizogenes agropine-type root-inducing (Ri) plasmid. However, the localization of GUS activity in the NgORF13 transgenic plants was similar to that in the RiORF13 transgenic plants. The GUS activity of NgORF13-GUS was high in genetic tumors cultured in vitro or developed spontaneously on F1 plants with aging or by wounding. The GUS activity in tumors was observed in bud primordia, vascular bundles and leaves in the buds. While the activity was lower than in tumors, NgORF13-GUS was also expressed in vascular bundles and the parenchymatous tissues in plants regenerated from tumors. Furthermore, the promoter activity of NgORF13 was induced by wounding and activated by exogenous application of methyl jasmonate. During tumorization, NgORF13 was induced at an early stage and showed expression patterns similar to both NgrolB and NgrolC whose expression were investigated by Nagata et al. (1996) Plant Cell Physiol. 37: 489-498. It is tho...Continue Reading

References

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Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·S Aoki, K Syno
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May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·F F WhiteE W Nester

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Citations

Oct 1, 2013·The Plant Cell·Momoko IkeuchiAkira Iwase
Jul 29, 2011·American Journal of Botany·Martina Talianova, Bohuslav Janousek
Oct 3, 2017·Frontiers in Plant Science·Ke Chen, Léon Otten

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