Expression analysis of the sunflower SF21 gene family reveals multiple alternative and organ-specific splicing of transcripts

Gene
Eduard LazarescuAndré Steinmetz

Abstract

The SF21 proteins were originally identified in sunflower pollen and in the stigmatic and transmitting tissues of sunflower pistils [Kräuter-Canham, R., Bronner, R., Evrard, J.L., Hahne, G., Friedt, W. and Steinmetz, A., 1997. A transmitting tissue- and pollen-expressed protein from sunflower with sequence similarity to the human RTP protein. Plant Science 129, 191-202.]. They are polypeptides of about 350 amino acids showing limited but significant sequence similarities with the animal NDR/RTP family of proteins of yet unknown function. Based on genomic sequence information derived from BAC clones containing SF21-related sequences we have identified transcripts generated from three different, but highly related genomic copies: SF21C, SF21D and SF21E. A sequence analysis of SF21C transcripts amplified by RT-PCR using specific primer pairs revealed a complex splicing pattern producing a minimum of three splice variant forms of the protein, one of 355 residues, and two truncated proteins of 90 and 138 amino acids, respectively. One of these variants was detected only in styles from pollinated florets, indicating organ-specific splicing. Two other splice variants, identified for a related transcript, SF21D, generate proteins diffe...Continue Reading

References

Jan 1, 1981·Annual Review of Biochemistry·R Breathnach, P Chambon
Sep 2, 1998·The Plant Cell·R W Sablowski, E M Meyerowitz
Feb 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·S P Dinesh-Kumar, B J Baker
Jun 22, 2000·Biochemical and Biophysical Research Communications·K L AgarwalaT Miyata
Aug 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sean A BeausoleilSteven P Gygi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.

Related Papers

Biochemical and Biophysical Research Communications
Jun-ichi KyunoMakoto Asashima
Biochemical and Biophysical Research Communications
Michael HunterLuba Kalaydjieva
The Prostate
Angelita G UtlegBiaoyang Lin
Current Opinion in Cell Biology
Emilie PerretEnrique Rodriguez-Boulan
© 2021 Meta ULC. All rights reserved