Expression and activity of nitric oxide synthases in human airway epithelium

American Journal of Respiratory Cell and Molecular Biology
D N WatkinsP J Thompson

Abstract

Nitric oxide (NO) synthesized by airway epithelium may be important in the regulation of airway inflammation and reactivity. As such, the expression and localization of nitric oxide synthase (NOS) isoforms was assessed in human airway tissue obtained following thoracotomy, and in cultured human airway epithelial (BEAS-2B) cells. NOS expression was determined by reverse transcription-polymerase chain reaction (RT-PCR)/Southern blot analysis, and localized by in situ hybridization and immunohistochemistry. No synthesis by cell cultures was detected by nitrite assay. Endothelial and neuronal constitutive NOS mRNAs were not detected in airways or cell cultures. Inducible NOS (iNOS) mRNA was detected in 5 of 6 airway specimens, and in situ hybridization demonstrated iNOS mRNA expression in columnar epithelial cells. This was confirmed by immunohistochemistry using an iNOS specific antibody. BEAS-2B cell cultures were stimulated with (I) combinations of tumor necrosis factor alpha (TNF alpha) (50-2,000 U/ml)/interferon gamma (IFN gamma) (20-500 U/ml)/lipopolysaccharide (LPS) (10 micrograms/ml) or (2) histamine (10(-3) M-10(-5) M). Cell cultures treated with TNF alpha/IFN gamma/LPS in combination expressed iNOS mRNA, and this was asso...Continue Reading

Citations

Oct 16, 2003·Nitric Oxide : Biology and Chemistry·Sarit OfferZecharia Madar
Nov 13, 2001·Allergy·M JorissenT Willems
Mar 27, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D N WatkinsP J Thompson
Mar 27, 2001·Immunology and Cell Biology·D Knight
Jan 8, 2004·Respirology : Official Journal of the Asian Pacific Society of Respirology·Darryl A Knight, Stephen T Holgate
Oct 16, 1999·Anesthesiology·W SteudelW M Zapol
Nov 17, 1998·American Journal of Respiratory and Critical Care Medicine·J G Widdicombe
Dec 10, 1999·American Journal of Respiratory and Critical Care Medicine·M KakuyamaP Vallance
Dec 10, 1999·American Journal of Respiratory and Critical Care Medicine·E GabbayT J Williams
Oct 13, 2000·American Journal of Respiratory and Critical Care Medicine·W SteudelW M Zapol
Feb 17, 2001·American Journal of Respiratory and Critical Care Medicine·Y J KaoG N Colasurdo
Jan 18, 2003·American Journal of Respiratory and Critical Care Medicine·Hartmut GrasemannFelix Ratjen
May 10, 2003·American Journal of Respiratory and Critical Care Medicine·Karin Storm van's GravesandeJeffrey M Drazen
May 5, 1999·American Journal of Respiratory Cell and Molecular Biology·A M HamadA J Knox
Jun 21, 2001·American Journal of Respiratory Cell and Molecular Biology·K UetaniS C Erzurum
Dec 13, 2006·American Journal of Respiratory Cell and Molecular Biology·Sandra M Wells, Andrij Holian
Apr 28, 2007·American Journal of Respiratory Cell and Molecular Biology·Tsutomu TamadaToshio Hattori
Feb 15, 2011·American Journal of Respiratory Cell and Molecular Biology·Erika N SutantoUNKNOWN Australian Respiratory Early Surveillance Team for Cystic Fibrosis
Jan 30, 2009·Chronic Respiratory Disease·G W RodwayJ M Sethi
Jan 15, 2011·Journal of Leukocyte Biology·David A WinkCarol A Colton
Jul 16, 2011·International Journal of Nanomedicine·J McCarthyMw Radomski
Jun 9, 2004·Treatments in Respiratory Medicine·Siobhan A Mulrennan, Anthony E Redington
Dec 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lance McNaughtonMarek W Radomski
Jul 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Vineet BhandariJack A Elias
Nov 4, 2015·Free Radical Biology & Medicine·Changjiang GuoAndrew Gow
May 20, 1999·Chest·C M Hart
Jul 25, 2012·Allergology International : Official Journal of the Japanese Society of Allergology·Mitsuru Munakata
Sep 27, 2016·Pulmonary Pharmacology & Therapeutics·Jatinder SinghDhandeep Singh
Oct 31, 2007·Pediatric Pulmonology·Ranjan SuriAndrew Bush
Apr 4, 2012·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Jacopo GalliGaetano Paludetti
Mar 17, 2004·Journal of Applied Physiology·Josephine HjobergEric S Silverman
Apr 26, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S RayP J Thompson
Jul 23, 2004·Physiological Reviews·Fabio L M RicciardoloGert Folkerts

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Bronchogenic

Bronchogenic Carcinoma is a malignant lung cancer arising in the epithelium of the bronchus or bronchiole. Discover the latest research on Bronchogenic Carcinoma here.

American Thoracic Association Journals

Discover the latest respiratory research published by the journals from the American Thoracic Society.

© 2021 Meta ULC. All rights reserved