Expression and biochemical analyses of the recombinant potato virus X 25K movement protein

FEBS Letters
N O KalininaJ G Atabekov

Abstract

The 25K movement protein (MP) of potato virus X (PVX) is encoded by the 5'-proximal gene of three overlapping MP genes forming a 'triple gene block'. The PVX 25K MP (putative NTPase-helicase) has been synthesized in Escherichia coli as a recombinant containing a six-histidine tag at the amino terminus. The His-tagged 25K protein was purified in a one-column Ni-chelate affinity chromatography procedure. In the absence of any other viral factors, this protein had obvious Mg2+-dependent ATPase activity, which was stimulated slightly (1.7-1.9-fold) by various polynucleotides. Like other viral proteins possessing ATPase-helicase motifs and many plant viral movement proteins, the PVX 25K MP was able to bind nucleic acids in vitro. The RNA binding activity of the 25K MP was pronounced only at very low salt concentrations and was independent of its ATPase activity.

References

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Citations

Oct 15, 2003·Journal of Molecular Biology·Nina P RodionovaJoseph G Atabekov
Apr 23, 2004·Trends in Plant Science·Dániel Silhavy, József Burgyán
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J G AtabekovN A Shapka
Feb 26, 2008·Biochemistry. Biokhimii︠a︡·A D LeshchinerN O Kalinina
Aug 24, 2005·FEBS Letters·Daria V RakitinaNatalia O Kalinina
Mar 1, 2003·Molecular Plant Pathology·Jeffrey S BattenCynthia Hemenway
Sep 1, 2007·Molecular Plant Pathology·Joseph AtabekovNina Rodionova
Oct 12, 2004·Plant Physiology and Biochemistry : PPB·Ken KobayashiAlejandro Mentaberry
Feb 13, 2001·The Journal of General Virology·O FedorkinS Yu Morozov
Oct 21, 2005·The Plant Journal : for Cell and Molecular Biology·Elizabeth H BayneDavid C Baulcombe
Jul 6, 2007·Virology·Timmy D SamuelsJeanmarie Verchot-Lubicz
Jul 1, 2004·The Journal of Biological Chemistry·Olga SamuilovaTimo Hyypiä
May 29, 2003·The Journal of General Virology·Sergey Yu Morozov, Andrey G Solovyev
Jul 22, 2005·The Journal of General Virology·M V SchepetilnikovS Yu Morozov

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