Expression and biochemical characterization of iron regulatory proteins 1 and 2 in Saccharomyces cerevisiae

Biochemistry
J D PhillipsElizabeth A Leibold

Abstract

Iron-regulatory proteins (IRPs) 1 and 2 are cytosolic RNA-binding proteins that bind to specific stem-loop structures, termed iron-responsive elements (IREs) that are located in the untranslated regions of specific mRNAs encoding proteins involved in iron metabolism. The binding of IRPs to IREs regulates either translation or stabilization of mRNA. Although IRP1 and IRP2 are similar proteins in that they are ubiquitously expressed and are negatively regulated by iron, they are regulated by iron by different mechanisms. IRP1, the well-characterized IRP in cells, is a dual-function protein exhibiting either aconitase activity when cellular iron is abundant or RNA-binding activity when cellular iron is scarce. In contrast, IRP2 lacks detectable aconitase activity and functions exclusively as an RNA-binding protein. To study and compare the biochemical characteristics of IRP1 and IRP2, we expressed wild-type and mutant rat IRP1 and IRP2 in the yeast Saccharomyces cerevisiae. IRP1 and IRP2 expressed in yeast bind the IRE RNA with high affinity, resulting in the inhibition of translation of an IRE-reporter mRNA. Mutant IRP2s lacking a 73 amino acid domain unique to IRP2 and a mutant IRP1 containing an insertion of this domain bound R...Continue Reading

Citations

Mar 27, 2001·Biochemical and Biophysical Research Communications·K P HoZ M Qian
Feb 24, 2001·Molecular Aspects of Medicine·P T LieuY Yang
Jul 3, 1998·Toxicology Letters·D M TempletonT Miralem
Sep 2, 2009·The Journal of Biological Chemistry·Mateen A KhanElizabeth C Theil
Jun 25, 2008·The Journal of Biological Chemistry·Michelle L WallanderElizabeth A Leibold
Feb 19, 2009·Molecular and Cellular Biology·Kimberly B ZumbrennenElizabeth A Leibold
May 11, 2000·Molecular and Cellular Biology·L T Jensen, V C Culotta
Aug 15, 2000·Annual Review of Nutrition·R S Eisenstein
Oct 16, 1999·The American Journal of the Medical Sciences·D J Haile
Jul 29, 2006·Biochimica Et Biophysica Acta·Michelle L WallanderRichard S Eisenstein
May 23, 1998·The Journal of Biological Chemistry·C BoutonJ C Drapier
Jun 20, 1998·The Journal of Biological Chemistry·L S GoesslingR E Thach
Jan 24, 1998·Critical Reviews in Clinical Laboratory Sciences·H A Goldenberg
Jul 12, 2003·Blood·Brian D Schneider, Elizabeth A Leibold
Feb 13, 1999·The Journal of Biological Chemistry·E S HansonE A Leibold
Apr 29, 1998·The Journal of Biological Chemistry·E S Hanson, E A Leibold
Aug 8, 1997·The Journal of Biological Chemistry·C BoutonJ C Drapier
May 12, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G MinottiG Cairo
Apr 6, 2001·Analytical Chemistry·F LisdatR Brigelius-Flohé

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