Expression and cell distribution of metabotropic glutamate receptor 5 in the rat cortex following traumatic brain injury

Brain Research
Jia-Wei WangZi-Xiang Cong

Abstract

Traumatic brain injury (TBI)-released excessive glutamate resulted in the activation of glutamate receptors including the metabotropic glutamate receptor 5 (mGluR5). To investigate the expression and cell distribution of mGluR5 in the rat cortex following TBI, western blot and quantitative real-time PCR were used to study the protein and mRNA level of mGluR5 respectively while immunohistochemistry analysis and double immunofluorescence with neural cell marker were used to define the cell distribution of mGluR5. Furthermore, we examined the effects of post-TBI administration of (R,S)-2-chloro-5-hydroxyphenylglycine (CHPG), a selective mGluR5 agonist, on the neuronal degeneration in the cortex. In the present study, we found that the protein level of mGluR5 was up-regulated by traumatic brain injury, while TBI-induced mGluR5 mRNA expression displayed biphasic changes with up-regulation in the early time and down-regulation in the late time after TBI. And neuron, astrocyte and microglia in the cortex after TBI all expressed mGluR5. Moreover, CHPG treatment significantly reduced the number of degenerating neurons detected by Fluoro-Jade C staining. These findings demonstrate that expression of mGluR5 differentially changes both spa...Continue Reading

References

Sep 1, 1990·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·P NilssonU Ungerstedt
Oct 1, 1995·Journal of Neurotrauma·R L HayesT K McIntosh
Aug 29, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·V BrunoF Nicoletti
Jul 16, 2002·Pharmacology, Biochemistry, and Behavior·Paul M LeaAlan I Faden
Jun 14, 2003·Brain : a Journal of Neurology·J J G GeurtsE Aronica
Nov 5, 2003·Experimental Neurology·Alison Berent Spillson, James W Russell
Apr 13, 2007·Brain Research·Giuseppa MudoDaniele F Condorelli
Dec 27, 2008·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Kimberly R ByrnesAlan I Faden
Apr 7, 2010·European Journal of Pharmacology·Agnes SimonyiGert R J Christoffersen
Mar 1, 2012·Journal of Neuroinflammation·Kimberly R ByrnesAlan I Faden

❮ Previous
Next ❯

Citations

Dec 20, 2013·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Weifeng SunChaodong Zhang
Mar 15, 2016·Frontiers in Integrative Neuroscience·Venkata S S S SajjaPamela J VandeVord
Dec 25, 2012·Biochemical and Biophysical Research Communications·Jia-Wei WangDing-Ding Zhang
Aug 9, 2013·Organic & Biomolecular Chemistry·Sven GottschalkAnurag Mishra
Dec 5, 2017·Experimental and Therapeutic Medicine·Limin Dou, Wenhua Yu
Dec 17, 2018·Molecular Neurobiology·Daniel YoungerNamas Chandra

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes in Repair & Regeneration

Astrocytes are glial cells found within the CNS and are able to regenerate new neurons. They become activated during CNS injury and disease. The activation leads to the transcription of new genes and the repair and regeneration of neurons. Discover the latest research on astrocytes in repair and regeneration here.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Astrocytes & Neurodegeneration

Astrocytes are important for the health and function of the central nervous system. When these cells stop functioning properly, either through gain of function or loss of homeostatic controls, neurodegenerative diseases can occur. Here is the latest research on astrocytes and neurodegeneration.

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.