Expression and Cell Distribution of SENP3 in Brain Tissue After Traumatic Brain Injury in Mice: A Pilot Study

Cellular and Molecular Neurobiology
Zhuang YuChun-Hua Hang

Abstract

SUMO-specific proteases 3 (SENP3) is a member of the small ubiquitin-like modifier-specific protease family and deconjugates SUMO2/3 from protein substrates. To date, the expression and function of SENP3 in traumatic brain injury (TBI) are unclear. The present study examined dynamic changes in SENP3 expression in the cerebral cortex and in its cellular localization, using an acute TBI model in adult mice. SENP3 expression was examined at 3, 6, 12, 24 h, 3, and 5 days after TBI using Western Blot analysis and quantitative real-time PCR. Immunohistochemistry and immunofluorescence were examined to detect SENP3 localization. Western Blot indicated that SENP3 protein levels gradually increased from 3 h after TBI and peaked at 24 h. Quantitative real-time PCR demonstrated a gradual increase in SENP3 expression, which peaked 12 h after TBI and declined subsequently. Immunohistochemical staining demonstrated that SENP3-positive cells were observed in both the sham and 24 h post-TBI groups. However, robust expression of SENP3 was seldom observed in the sham group, while it was notably enhanced after TBI. Furthermore, immunofluorescence results revealed that the expression of SENP3 increased more significantly in neurons at day 1 after ...Continue Reading

References

Apr 13, 2006·The Journal of Biological Chemistry·Limin Gong, Edward T H Yeh
May 15, 2007·Trends in Biochemical Sciences·Debaditya Mukhopadhyay, Mary Dasso
Nov 8, 2007·Nature Reviews. Neuroscience·Stéphane MartinJeremy M Henley
Dec 18, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Eugene ParkAndrew J Baker
Apr 7, 2009·The European Journal of Neuroscience·Marco FeligioniJeremy M Henley
Aug 29, 2009·Nature Protocols·Michael A FlierlEsther Shohami
May 14, 2010·The Biochemical Journal·Kevin A Wilkinson, Jeremy M Henley
Jul 28, 2010·Journal of Neuroinflammation·Kenji DohiTohru Aruga
Feb 22, 2011·Journal of Neurotrauma·Atsuhiro NakagawaTeiji Tominaga
Aug 25, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Anna Lena DatwylerChristoph Harms
Oct 12, 2012·Neurochemical Research·Haixiang WeiJian Zhao
Feb 28, 2013·Nature Reviews. Neurology·Bob RoozenbeekDavid K Menon
Dec 10, 2013·Nature·Theodore L RothDorian B McGavern
Jun 9, 2014·Journal of Molecular Neuroscience : MN·Kazuyuki MiyamotoSeiji Shioda

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