Expression and cellular distribution of cyclin-dependent kinase 4 (Cdk4) and connexin 43 (Cx43) in porcine oocytes before and after in vitro maturation

Acta Veterinaria Hungarica
Bartosz KempistyMaciej Zabel

Abstract

It is recognised that connexin 43 (Cx43) and cyclin-dependent kinase 4 (Cdk4) are involved in the cumulus cell-oocyte communication via gap junctions and the control of cell cycle progress. However, little is known about their mRNA expression pattern and encoded proteins distribution in porcine oocytes during in vitro maturation (IVM). Cumulus-oocyte complexes (COCs) were collected from 31 puberal crossbred Landrace gilts and analysed for their Cdk4 and Cx43 mRNA expression using RQ-PCR and for the respective protein expression by confocal microscopic observations. An increased Cdk4 and Cx43 mRNA expression was found in oocytes after IVM (P < 0.001 and P < 0.05, respectively). Confocal microscopic observations revealed a significant increase of Cdk4 protein expression in the cytoplasm of oocytes during the maturation process. The localisation of Cx43 changed from zona pellucida before to cytoplasm of oocytes after IVM. It is supposed that the increased expression of Cdk4 and Cx43 mRNA in oocytes after IVM is linked with the accumulation of a large amount of templates during the process of oocyte maturation. The translocation especially of Cx43 from the zona pellucida into the cytoplasm may be associated with a decrease in gap j...Continue Reading

References

Sep 1, 1986·Journal of Reproduction and Fertility·Z Polański
Sep 1, 1993·Molecular Reproduction and Development·G ValdimarssonG M Kidder
Jan 5, 2000·Journal of Biotechnology·O ThellinE Heinen
Apr 20, 2004·Cardiovascular Research·Goran Söhl, Klaus Willecke
Feb 8, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Disha PantAnna T Grazul-Bilska
May 9, 2006·Developmental Cell·Cyril BerthetPhilipp Kaldis
May 9, 2008·Reproduction, Fertility, and Development·Bartosz KempistyPaweł P Jagodziński
Apr 9, 2009·Reproductive Biology·Bartosz KempistyPaweł P Jagodziński
Apr 9, 2009·Reproductive Biology·Marta JackowskaJedrzej M Jaśkowski
Mar 24, 2011·Reproduction, Fertility, and Development·Sylvie Chastant-MaillardKarine Reynaud
Sep 3, 2011·Molecular Reproduction and Development·Heide Schatten, Qing-Yuan Sun
Feb 4, 2012·Journal of the National Cancer Institute·Patrick J RobertsNorman E Sharpless
Apr 3, 2012·Annual Review of Biochemistry·Qi Qun Tang, M Daniel Lane
Jun 14, 2012·The Journal of Biological Chemistry·Jingna WangYouwei Zhang

❮ Previous
Next ❯

Citations

May 14, 2016·Zygote : the Biology of Gametes and Early Embryos·Seunghoon LeeEimei Sato
Jul 25, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Joanna BudnaBartosz Kempisty

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.