PMID: 2111957Jun 1, 1990Paper

Expression and characterization of a functional human immunodeficiency virus envelope glycoprotein in insect cells

Virology
D E Wells, R W Compans

Abstract

Recombinant baculoviruses were used to express the gp160 envelope glycoprotein of the human immunodeficiency virus type 1 (HIV-1) and a truncated variant designated gp160(t) which lacks a transmembrane domain. Glycosylation, proteolytic cleavage, secretion, and biological activities of gp160 and gp160(t) 160(t) were studied in Spodoptera frugiperda cells. Both proteins were rapidly glycosylated and initially were found to be totally endo-beta-N-acetyl-D-glucosaminidase H (endo-H) sensitive. However, partial resistance to endo-H was gradually acquired by both molecules. gp160 was found to remain cell-associated, whereas gp160(t) was secreted into the culture medium in large amounts. A fraction of gp160 and gp160(t) appeared to be proteolytically cleaved, and a cleavage product corresponding in size to gp120 was identified in the culture medium. gp160(t) was found to interact specifically with CD4 receptors without any requirement for proteolytic cleavage. The gp160 protein was shown to be expressed on the surface of S. frugiperda cells by direct immunofluorescence. These surface molecules were biologically active, as demonstrated by their ability to induce syncytium formation when cocultivated with HeLa T4 cells.

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Citations

Jan 1, 1996·Cytotechnology·A R BernardP Graber
Nov 7, 2000·Biochimica Et Biophysica Acta·M Moulard, E Decroly
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y LiC Y Kang
May 1, 1995·The Journal of Experimental Medicine·C G ParkG M Winslow
Mar 10, 2000·AIDS Research and Human Retroviruses·Q YaoC Chen
Oct 1, 1993·AIDS Research and Human Retroviruses·N L PaulR A Weiss
Feb 1, 1992·Immunobiology·S Norley, R Kurth
Jun 25, 2005·Journal of Medical Virology·Sibusiso P NkosiMaria A Papathanasopoulos
Aug 31, 2010·Journal of Molecular Biology·Leopold KongQuentin J Sattentau
Dec 31, 2009·PLoS Pathogens·Yuntao Wu, Alyson Yoder

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