Expression and characterization of a novel enantioselective lipase from Acinetobacter species SY-01

Biochimie
Soo-Jin HanYe Sun Han

Abstract

A novel lipase gene, lipase A, of Acinetobacter species SY-01 (A. species SY-01) was cloned, sequenced, and expressed in Bacillus subtilis 168. The deduced amino acid (aa) sequences for the lipase A and its chaperone, lipase-specific chaperone, were found to encode mature proteins of 339 aa (37.2 kDa) and 347 aa (38.1 kDa), respectively. The aa sequence of lipase A and lipase-specific chaperone shared high homology 82 and 67% identity with the lipase A and the lipase B of A. species RAG-1. This new lipase was defined as a group I Proteobacterial lipase family. The expressed lipase A was purified through sequential treatment with Q-Sepharose, Resource Q, and Superdex-S75 columns. The maximal activity was observed at 50 degrees C for hydrolysis of p-nitrophenyl monoesters and found to be stable at pH 9-11, with optimal activity at pH 10. Lipase A hydrolyzed wide range of fatty acid esters of p-nitrophenyl, but preferentially hydrolyzed short length acyl chains (C2 and C4). Moreover, lipase A from A. species SY-01 catalyzed hydrolysis of the two acetate isomers of cis-(+/-)-2-(bromomethyl)-2-(2,4-dichloro phenyl)-1,3-dioxolane-4-methyl acetate, an intermediate required for the synthesis of Itraconazole which was an anti-fungal dru...Continue Reading

References

Dec 1, 1992·Applied and Environmental Microbiology·L G FrenkenC T Verrips
Jul 1, 1992·Journal of General Microbiology·S WohlfarthU K Winkler
Aug 1, 1992·Archives of Biochemistry and Biophysics·M Chihara-SiomiJ Oda
Apr 1, 1989·Trends in Biochemical Sciences·J Harwood
Dec 1, 1994·Molecular & General Genetics : MGG·J L AamandD J McConnell
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A H HobsonD J McConnell
Aug 1, 1993·Enzyme and Microbial Technology·E J Gilbert
Sep 1, 1994·FEMS Microbiology Reviews·K E JaegerO Misset
Jan 1, 1994·Critical Reviews in Microbiology·G Soberón-Chávez, B Palmeros
Aug 1, 1993·Molecular Microbiology·L G FrenkenC T Verrips
Sep 23, 1998·Trends in Biotechnology·K E Jaeger, M T Reetz
Apr 13, 2000·Journal of Bacteriology·S AshikagaF Kawamura
Aug 19, 2000·Microbiological Research·J Pratuangdejkul, S Dharmsthiti
Aug 29, 2001·FEMS Microbiology Reviews·K H van WelyA J Driessen
Jul 16, 2002·Biochimica Et Biophysica Acta·Hyung Kwoun KimTae Kwang Oh
Nov 26, 2002·European Journal of Biochemistry·Erick A SnellmanRita R Colwell

❮ Previous
Next ❯

Citations

Feb 22, 2011·Applied Microbiology and Biotechnology·Xiaomei ZhengYunliu Fan
Jul 28, 2007·Extremophiles : Life Under Extreme Conditions·Hiroyasu OginoHaruo Ishikawa
Sep 21, 2004·Journal of Industrial Microbiology & Biotechnology·Erick A Snellman, Rita R Colwell
May 9, 2012·Applied Biochemistry and Biotechnology·Debamitra ChakravortySanjukta Patra
Sep 8, 2012·Applied Biochemistry and Biotechnology·Saisubramanian Nagarajan
May 29, 2008·Journal of Basic Microbiology·Ramesh Chand KasanaSudesh Kumar Yadav
Feb 20, 2015·Applied Microbiology and Biotechnology·Jaejoon Jung, Woojun Park
Sep 5, 2009·Biotechnology Progress·Takuya Kawata, Hiroyasu Ogino
Nov 29, 2013·Biotechnology and Bioengineering·Hyun Tae HwangArvind Varma
Oct 12, 2018·Anais Da Academia Brasileira De Ciências·Graciane FuriniJosé C Germani
Jul 7, 2015·PloS One·Chao RanZhigang Zhou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.