Expression and characterization of a recombinant i-type lysozyme from the harlequin ladybird beetle Harmonia axyridis

Insect Molecular Biology
A BeckertA Vilcinskas

Abstract

Lysozymes are enzymes that destroy bacterial cell walls by hydrolysing the polysaccharide component of peptidoglycan. In insects, there are two classes of lysozymes, the c-type with muramidase activity and the i-type whose prototypical members from annelids and molluscs possess both muramidase and isopeptidase activities. Many insect genes encoding c-type and i-type lysozymes have been identified during genome and transcriptome analyses, but only c-type lysozymes have been functionally characterized at the protein level. Here we produced one of five i-type lysozymes represented in the immunity-related transcriptome of the invasive harlequin ladybird beetle Harmonia axyridis as recombinant protein. This was the only one containing the serine and histidine residues that are thought to be required for isopeptidase activity. This i-type lysozyme was recombinantly expressed in the yeast Pichia pastoris, but the purified protein was inactive in both muramidase and isopeptidase assays. Transcription and immunofluorescence analysis revealed that this i-type lysozyme is produced in the fat body but is not inducible by immune challenge. These data suggest that i-type lysozymes in insects may have acquired novel and as yet undetermined fu...Continue Reading

References

May 1, 1975·European Journal of Biochemistry·J Jollès, P Jollès
Feb 1, 1991·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·I P Baskova, G I Nikonov
Mar 17, 2000·Biochimica Et Biophysica Acta·L L ZavalovaE D Sverdlov
Apr 20, 2002·Journal of Molecular Evolution·Sana BachaliJean S Deutsch
Jul 24, 2002·Nucleic Acids Research·Kazutaka KatohTakashi Miyata
Feb 28, 2003·Biology of Reproduction·Arabinda MandalJohn C Herr
Aug 13, 2003·Bioinformatics·Fredrik Ronquist, John P Huelsenbeck
Aug 28, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Ørjan M OlsenBjørnar Myrnes
Oct 3, 2003·Cellular and Molecular Life Sciences : CMLS·K TakeshitaT Imoto
Apr 22, 2008·Nucleic Acids Research·A DereeperO Gascuel
Dec 17, 2008·Insect Molecular Biology·B Altincicek, A Vilcinskas
Dec 23, 2008·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·F C CançadoS R Marana
May 21, 2009·Developmental and Comparative Immunology·Radka JoskováMartin Bilej
Nov 26, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mayur K KajlaSusan M Paskewitz
Apr 24, 2010·Journal of Biosciences·Lien Callewaert, Chris W Michiels
Sep 8, 2010·Fish & Shellfish Immunology·Hong-Wei ZhangJin-Xing Wang
Nov 17, 2010·Biochemistry. Biokhimii︠a︡·L L ZavalovaI P Baskova
Jun 17, 2011·BMC Evolutionary Biology·David M IrwinCaro-Beth Stewart
May 15, 2012·Journal of Biosciences·Joris M Van Herreweghe, Chris W Michiels
Nov 23, 2012·Proceedings. Biological Sciences·Andreas VilcinskasHeiko Vogel
Jan 5, 2013·The Journal of Biological Chemistry·Binh V LeRichard H G Baxter
Apr 3, 2013·Developmental and Comparative Immunology·Ulrich TheopoldMitchell S Dushay
Mar 13, 2014·Insect Biochemistry and Molecular Biology·Peter M CampbellTara D Sutherland
Dec 6, 2014·Developmental and Comparative Immunology·Annika BeckertAndreas Vilcinskas

❮ Previous
Next ❯

Citations

Oct 4, 2016·Developmental and Comparative Immunology·Heiko VogelAndreas Vilcinskas
May 10, 2017·Nature Communications·Heiko VogelAndreas Vilcinskas
Oct 22, 2019·Developmental and Comparative Immunology·Matan ShelomiHeiko Vogel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.