PMID: 8948435Jun 15, 1995Paper

Expression and characterization of a recombinant C4b-binding protein lacking the beta-chain

The Biochemical Journal
Y HärdigB Dahlbäck

Abstract

C4b-binding protein (C4BP) is a high-molecular-mass glycoprotein which contains binding sites for complement component C4b, anti-coagulant vitamin K-dependent protein S and serum amyloid P component (SAP). The major form of C4BP in plasma is composed of seven identical alpha-chains and a single beta-chain. We have expressed full-length cDNA for the alpha-chain in a eukaryotic expression system and characterized functional properties of non-beta-chain-containing C4BP. During synthesis, recombinant alpha-chains polymerized into two different high-molecular-mass C4BP forms which were composed of seven or eight alpha-chains. Recombinant C4BP bound C4(H2O) (used instead of C4b) equally as well as native C4BP, functioned equally as well as factor I cofactor in the degradation of C4(H2O) and bound to SAP. In contrast, the recombinant C4BP did not bind protein S and therefore did not inhibit the ability of protein S to function as a cofactor to activated protein C. Tunicamycin treatment of the transfected cells prevented N-linked glycosylation, but did not affect polymerization of the alpha-chains into a high-molecular-mass C4BP. The non-glycosylated C4BP had comparable properties to glycosylated C4BP in several functional assays. Thes...Continue Reading

Citations

Nov 10, 1995·The Journal of Biological Chemistry·P García de FrutosB Dahlbäck
Aug 23, 1996·The Journal of Biological Chemistry·Y Härdig, B Dahlbäck
Jan 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S OudinJ H Cohen
Aug 10, 2010·The Journal of Biological Chemistry·Sofia Carlsson, Björn Dahlbäck
Feb 26, 2010·Thrombosis Research·Makoto IkejiriMasaki Itoh

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.