Expression and characterization of a thermostable penicillin G acylase from an environmental metagenomic library

Biotechnology Letters
Qian ZhangRunying Zeng

Abstract

One clone (ACPGA001) exhibiting penicillin G acylase (PGA) activity was screened from a metagenomic library by using a medium containing penicillin G. A novel PGA gene from the inserted fragment of ACPGA001 was obtained by sequencing. The amino acid sequence of ACPGA001 PGA exhibited <33 % similarity to PGAs retrieved from GenBank. This gene was expressed in Escherichia coli M15 and the recombinant protein was purified and characterized. The ACPGA001 PGA exhibited a maximum activity at 60 °C and showed high activity at pH 4-10 with an optimum pH of 8.0. This enzyme was stable at 40 °C for 70 min with a half-life of 60 min at 55 °C. These beneficial characteristics of ACPGA001 PGA provide some advantages for the potential application of ACPGA001 PGA in industry.

References

Jan 1, 1992·DNA Sequence : the Journal of DNA Sequencing and Mapping·G LjubijankićV Glisin
Jul 25, 1986·Nucleic Acids Research·G SchumacherA Böck
Nov 1, 1988·Applied and Environmental Microbiology·H OhashiM Yano
Jan 1, 1967·Journal of Bacteriology·C Chiang, R E Bennett
Mar 1, 1995·Protein Science : a Publication of the Protein Society·H E KleiJ A Kelly
Jan 19, 1995·Nature·H J DugglebyP C Moody
Feb 24, 2001·Protein Science : a Publication of the Protein Society·C Oinonen, J Rouvinen
Sep 11, 2004·Current Opinion in Biotechnology·Thomas R M BarendsBauke W Dijkstra
Jul 5, 2007·Journal of Bioscience and Bioengineering·Jeyaprakash Rajendhran, Paramasamy Gunasekaran
Dec 19, 2007·Extremophiles : Life Under Extreme Conditions·Meixiang XuFengping Wang
Sep 22, 2009·Journal of Bacteriology·Hyun-Myung OhJang-Cheon Cho
Nov 12, 2010·Extremophiles : Life Under Extreme Conditions·Yingjing ZhangRunying Zeng
Aug 17, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Jeong Ho JeonJung-Hyun Lee
Nov 5, 2011·Extremophiles : Life Under Extreme Conditions·Xiao Ping HuDon Cowan
Jul 5, 2012·Extremophiles : Life Under Extreme Conditions·Tracy L MeiringDon A Cowan
Mar 1, 1994·World Journal of Microbiology & Biotechnology·B S DeshpandeJ G Shewale

❮ Previous
Next ❯

Citations

Nov 13, 2019·Current Pharmaceutical Biotechnology·Luis Cobos-PucJosé Luis Martínez Hernández
Nov 16, 2019·Current Pharmaceutical Biotechnology·Chih-Wen FangPao-Chu Wu
Sep 10, 2020·Folia Microbiologica·Martina BorčinováPavel Kyslík
Oct 14, 2017·FEMS Microbiology Letters·Francesca BeriniFlavia Marinelli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Polyendocrinopathies

Autoimmune polyendocrinopathies, also called polyglandular autoimmune syndromes (PGASs), or polyendocrine autoimmune syndromes(PASs), are a heterogeneous group of rare diseases characterized by autoimmune activity against more than one endocrine organ, although non-endocrine organs can be affected. Discover the latest research on autoimmune polyendocrinopathies here.

Autoimmune Polyendocrine Syndromes

This feed focuses on a rare genetic condition called Autoimmune Polyendocrine Syndromes, which are characterized by autoantibodies against multiple endocrine organs. This can lead to Type I Diabetes.