Expression and characterization of recombinant murine lactoferrin

Gene
Pauline P WardO M Conneely

Abstract

Lactoferrin (Mw=78 kDa) is a member of the transferrin family of iron-binding glycoproteins. Previous studies carried out primarily in vitro indicate that the protein has multifunctional properties and may be involved in regulation of iron homeostasis, inhibition of bacterial growth and regulation of immune responses. However, the significance and species specificity of these proposed functions in vivo have not been adequately addressed due to lack of sufficient purified homospecies lactoferrin for analysis in small animal models. We previously reported the successful production of biologically active recombinant human lactoferrin using an Aspergillus expression system. In the present study, we report the production of recombinant murine lactoferrin using a similar expression strategy. Recombinant murine lactoferrin was purified to homogeneity and was similar in size and immunoreactivity to native murine milk lactoferrin. The recombinant protein was correctly processed at its N-terminus and was glycosylated. Interestingly, while both human and murine lactoferrin bind iron in a 2:1 molar ratio, iron bound to recombinant murine lactoferrin was more acid labile than human lactoferrin, demonstrating species-specific variation in th...Continue Reading

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Citations

Oct 3, 2002·Protein Expression and Purification·M ParamasivamA Srinivasan
May 16, 2000·Immunology·M CumberbatchI Kimber
Oct 18, 2001·Journal of the American College of Nutrition·O M Conneely
May 11, 2010·Applied and Environmental Microbiology·José Miguel P Ferreira de OliveiraLeo H de Graaff
Dec 17, 2002·Molecular and Cellular Biology·Pauline P WardOrla M Conneely
Mar 23, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Pauline P WardOrla M Conneely
Feb 5, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Jiarong WangJianhua Wang
Aug 25, 2005·Expert Review of Vaccines·Stephen J Streatfield
Sep 1, 2015·Archivum Immunologiae Et Therapiae Experimentalis·Ivonne Maciel Arciniega-MartínezAldo Arturo Reséndiz-Albor
Nov 13, 2007·Journal of Food Science·Gen-Hung ChenShann-Tzong Jiang
Dec 7, 2007·Protein Expression and Purification·Jie ZhangGuoxiang Cheng
Sep 27, 2019·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Surender SinghJai K Kaushik
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cristina GuillénJeremy H Brock
Mar 26, 2021·International Immunopharmacology·Michał ZimeckiMarian L Kruzel

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