PMID: 2105101Jan 30, 1990Paper

Expression and characterization of the intestinal Na+/glucose cotransporter in COS-7 cells

Biochimica Et Biophysica Acta
B BirnirE M Wright

Abstract

Cells derived from the simian kidney, COS-7 cells, were transfected with a eucaryotic expression vector (pEUK-C1) containing the clone for the rabbit intestinal Na+/glucose cotransporter. Expression was monitored after transfection with lipofectin by measuring the initial rate of alpha-methylglucopyranoside (MeGlc) uptake. Cells transfected with vector containing the cDNA for the Na+/glucose cotransporter expressed Na(+)-dependent MeGlc transport. Neither control cells nor cells transfected with vector lacking cloned cDNA expressed the cotransporter. Na(+)-dependent MeGlc uptake into transfected cells was saturable (Km 150 microM), phlorizin-sensitive (Ki 11 microM), and inhibited by sugar analogs (D-glucose greater than MeGlc greater than D-galactose greater than 3-O-methyl-D-glucoside greater than D-allose much greater than L-glucose). Europium was able to mimic Na+ in driving MeGIC uptake. Finally, tunicamycin, an inhibitor of asparagine-linked glycosylation, inhibited the expression of Na(+)-dependent MeGlc transport 80%. We conclude that the rabbit intestinal Na+/glucose cotransporter expressed in COS-7 cell exhibits very similar kinetic properties to that in the native brush border and to that expressed in Xenopus oocytes...Continue Reading

References

Aug 1, 1989·The Journal of Membrane Biology·T S IkedaE M Wright
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P L FelgnerM Danielsen
Jan 1, 1984·Annual Review of Physiology·B R StevensE M Wright

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Citations

Mar 1, 1991·Pflügers Archiv : European journal of physiology·B BirnirE M Wright
Feb 1, 1996·Pflügers Archiv : European journal of physiology·A M Pajor, H G Valmonte
Aug 1, 1992·Current Opinion in Cell Biology·E M WrightE Turk
Nov 1, 1991·The Journal of Clinical Investigation·E M WrightJ Dyer
May 7, 1991·Biochimica Et Biophysica Acta·M A HedigerE M Wright
Jun 22, 1994·Biochimica Et Biophysica Acta·J S Wu, J E Lever
Oct 11, 2003·FEBS Letters·Ida LagerSylvie Lalonde
May 30, 2015·Nutrition Reviews·Danielle M StringerCarla G Taylor
Mar 31, 2017·American Journal of Physiology. Renal Physiology·Blythe D Shepard, Jennifer L Pluznick
Apr 30, 2011·Physiological Reviews·Ernest M WrightBruce A Hirayama
Sep 1, 1996·DNA and Cell Biology·S LambotteH Koepsell
Jan 3, 2001·American Journal of Physiology. Renal Physiology·E M Wright
Aug 1, 1991·The American Journal of Physiology·B A HirayamaE M Wright
Sep 1, 1994·Infection and Immunity·R G Russell, D C Blake
Aug 8, 2021·Drugs·Lucia Del VecchioSimonetta Genovesi

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