Expression and Characterization of Windmill Palm Tree (Trachycarpus fortunei) Peroxidase by Pichia pastoris

Journal of Agricultural and Food Chemistry
Boting WenQing X Li

Abstract

Currently, commercial plant peroxidases are all native and are isolated from plants such as horseradish and soybean. No recombinant plant peroxidase products have been available on the commercial market. The gene encoding peroxidase was cloned from windmill palm tree leaves. The codon-optimized gene was transformed into Pichia pastoris for expression. The recombinant windmill palm tree peroxidase (rWPTP) expressed by P. pastoris showed high stability under pH 2-10 and temperatures up to 70 °C to many metallic salts and organic solvents. The substrate specificity of WPTP was determined, and among the substrates tested, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) was most suitable for WPTP. The Michaelis constants with the substrates H2O2 and ABTS were 4.6 × 10(-4) and 1.6 × 10(-4) M, respectively. The rWPTP expressed in P. pastoris may be a suitable enzyme for the biosynthesis of polymers because of its high stability and activity under acidic conditions.

References

Apr 15, 1987·Analytical Chemistry·R Q Thompson
Dec 24, 1997·Nature Structural Biology·M GajhedeT L Poulos
Jan 21, 2000·FEMS Microbiology Reviews·J L Cereghino, J M Cregg
Jul 31, 2002·Biochimica Et Biophysica Acta·Ivan Yu Sakharov, Irina V Sakharova
Oct 18, 2003·Applied Microbiology and Biotechnology·Y-S Keum, Q X Li
Apr 28, 2004·Chemosphere·Young Soo Keum, Qing X Li
Oct 26, 2005·Biosensors & Bioelectronics·Inna S AlpeevaIvan Yu Sakharov
Dec 21, 2006·Journal of Agricultural and Food Chemistry·Alexei V CaramyshevIvan Yu Sakharov
Jul 17, 2007·Biomacromolecules·Alexei V CaramyshevIvan Yu Sakharov
Jul 1, 1987·Biotechnology and Bioengineering·J S DordickA M Klibanov
Aug 30, 2008·Biochimie·Laura S ZamoranoValery L Shnyrov
May 12, 2009·International Journal of Biological Macromolecules·Laura S ZamoranoValery L Shnyrov
Oct 3, 2012·Protein Expression and Purification·Oliver SpadiutChristoph Herwig
Nov 11, 2014·Journal of Agricultural and Food Chemistry·Margaret R BakerQing X Li

❮ Previous
Next ❯

Citations

Jun 7, 2019·Acta Biochimica Et Biophysica Sinica·Xiaodong CuiZhuanhua Wang
Apr 13, 2018·Journal of Agricultural and Food Chemistry·Bo FuQing X Li
Nov 20, 2018·Inorganic Chemistry·Andrea LiberatoManuel G Basallote

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Agricultural and Food Chemistry
Alexei V CaramyshevIvan Y Sakharov
Bulletin De La Société De Chimie Biologique
P DUQUENOIST GUTH
Biochemistry. Biokhimii︠a︡
I Yu SakharovI V Sakharova
© 2022 Meta ULC. All rights reserved