Expression and contribution of the HIF-1α/VEGF signaling pathway to luteal development and function in pregnant rats

Molecular Medicine Reports
Lixiang WuZhengchao Wang

Abstract

Vascular endothelial growth factor (VEGF) is vital in normal and abnormal angiogenesis in the ovary, particularly during the early development of the corpus luteum in the ovary. However, the molecular regulation of the expression VEGF during luteal development in vivo remains to be fully elucidated. As the expression of VEGF is mediated by hypoxia‑inducible factor (HIF)‑1α in luteal cells cultured in vitro, determined in our previous study, the present study was performed to confirm the hypothesis that HIF‑1α is induced and then regulates the expression of VEGF and VEGF‑dependent luteal development/function in vivo. This was investigated using a pregnant rat model treated with a small‑molecule inhibitor of HIF‑1α, echinomycin (Ech). The development of the corpus luteum in the pregnant rat ovary was identified via performing assays of the serum progesterone, testosterone and estradiol concentrations by radioimmunoassay, accompanied with determination of the changes in the expression levels of HIF‑1α and VEGF by reverse transcription‑quantitative polymerase chain reaction at different days of the developmental process. On day 5, serum progesterone levels were markedly increased, whereas serum levels of testosterone and estradiol ...Continue Reading

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Citations

Mar 3, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Xiaowei LuYun Feng
Feb 5, 2021·Frontiers in Cell and Developmental Biology·Zonghao TangZhengchao Wang
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Dody Houston Billhaq, Seunghyung Lee

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