Expression and copy number gains of the RET gene in 631 early and mid stage non-small cell lung cancer cases

Thoracic Cancer
Ling TanHao Tang

Abstract

To identify whether RET is a potential target for NSCLC treatment, we examined the status of the RET gene in 631 early and mid stage NSCLC cases from south central China. RET expression was identified by Western blot. RET-positive expression samples were verified by immunohistochemistry. RET gene mutation, copy number variation, and rearrangement were analyzed by DNA Sanger sequencing, TaqMan copy number assays, and reverse transcription-PCR. ALK and ROS1 expression levels were tested by Western blot and EGFR mutation using Sanger sequencing. The RET-positive rate was 2.5% (16/631). RET-positive expression was related to poorer tumor differentiation (P < 0.05). In the 16 RET-positive samples, only two samples of moderately and poorly differentiated lung adenocarcinomas displayed RET rearrangement, both in RET-KIF5B fusion partners. Neither ALK nor ROS1 translocation was found. The EGFR mutation rate in RET-positive samples was significantly lower than in RET-negative samples (P < 0.05). RET-positive expression in early and mid stage NSCLC cases from south central China is relatively low and is related to poorer tumor differentiation. RET gene alterations (copy number gain and rearrangement) exist in all RET-positive samples. RE...Continue Reading

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Citations

Aug 2, 2018·Cancer Biology & Therapy·Min LiCheng-Ping Hu
Jan 23, 2019·Frontiers in Physiology·Lois M Mulligan
Oct 24, 2019·Arquivos Brasileiros De Cirurgia Digestiva : ABCD = Brazilian Archives of Digestive Surgery·Abdolhamid AmooeeHossein Neamatzadeh
Aug 21, 2020·Cell and Tissue Research·Kumi Kawai, Masahide Takahashi
Feb 23, 2020·Journal of Mammary Gland Biology and Neoplasia·Albana GattelliSabrina A Vallone
Jul 16, 2020·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Carmen BelliGiuseppe Curigliano
Jan 19, 2021·Annals of Oncology : Official Journal of the European Society for Medical Oncology·C BelliG Curigliano
Mar 9, 2020·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Serisha MoodleyLois M Mulligan

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Methods Mentioned

BETA
PCR
Genotyping

Software Mentioned

CopyCaller
SPSS

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