PMID: 30141608Dec 1, 2016Paper

Expression and Detection of Lentivirus-mediated Green Fluorescent Protein in Schistosoma japonicum

Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
Yue XinWei Hu

Abstract

To test if the green fluorescent protein gene can be expressed in Schistosoma japonicum and produce green fluorescence signals there. The spontaneous fluorescence in Schistosoma japonicum at different developmental stages was observed by fluorescence microscopy. The cultured 14-day schistosomula were infected with the lentiviral vector containing cyto megalo virus (CMV)-driven zsGreen gene, or were incubated with RPMI 1640 medium containing 10% fetal bovine serum as a negative control. At 48 h after infection, genomic DNA and total RNA were extracted, and cDNA was synthesized. PCR was performed to detect the genomic integration of zsGreen gene and the expression of zsGreen mRNA. Green fluorescence was observed under a fluorescence microscope at 24 h and 48 h after infection, and at various time points after replacement with fresh culture medium. There was no obvious spontaneous fluorescence in schistosomula, but the adult worms showed clear spontaneous fluorescence. PCR results showed a specific band of 173 bp from the schistosomula genomic DNA, which corresponded to the zsGreen gene. Gene sequencing also confirmed this result. At 24 h after infection, green fluorescence was seen in the intestine of schistosomula, which was, ho...Continue Reading

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Related Papers

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Qinghua QiaoWen Yin
In Vitro Cellular & Developmental Biology. Animal
G E Moore, D B Hood
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
Linnan WangChunguang Zhou
© 2022 Meta ULC. All rights reserved