Expression and developmental regulation of the k-FGF oncogene in human and murine embryonal carcinoma cells.

In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
J Tiesman, A Rizzino

Abstract

Embryonal carcinoma (EC) cells provide an effective model system for studying growth factor production and regulation during mammalian embryogenesis. Our earlier data indicated that the mouse EC cell lines F9 and PC-13 and the human EC cell line NT2/D1 produce a factor with properties similar to those ascribed to members of the fibroblast growth factor (FGF) family and that production of this FGF-related factor is suppressed when all three EC cell lines are induced to differentiate. Subsequent studies suggested that NT2/D1 EC cells express transcripts for basic FGF (bFGF). The current study confirms and extends these findings using a combination of reverse transcription and polymerase chain reaction (RT-PCR). In this study, the expression of bFGF and other members of the FGF family have been examined in F9 and PC-13 cells in addition to NT2/D1 EC cells. In contrast to NT2/D1 EC cells, bFGF expression could not be detected in F9 and PC-13 EC cells. Additionally, expression of four other members of the FGF family (acidic FGF, int-2, FGF-5, and FGF-6) were not detected in NT2/D1, F9, or PC-13 EC cells. However, expression of another member of the FGF family, the k-FGF oncogene, was detected in NT2/D1, F9, and PC-13 EC cells. Moreo...Continue Reading

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Citations

Dec 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·W J CampbellA Rizzino
Oct 1, 1996·In Vitro Cellular & Developmental Biology. Animal·K Miller, A Rizzino
Nov 1, 1992·Developmental Biology·Y G MaA Rizzino
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Jan 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·W J CampbellA Rizzino
Sep 1, 1994·Molecular Reproduction and Development·A Rizzino, E Rosfjord
May 17, 2000·Molecular Reproduction and Development·D L Kelly, A Rizzino
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Dec 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J Tiesman, A Rizzino
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Aug 1, 1996·Molecular Reproduction and Development·K LambA Rizzino
Dec 1, 1991·Mechanisms of Development·J Schoorlemmer, W Kruijer

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