Expression and distribution of antimicrobial peptides in the skin of healthy beagles

Veterinary Dermatology
Domenico SantoroKaren L Campbell

Abstract

Antimicrobial peptides (AMPs) are small proteins involved in defense against pathogenic organisms. Defensins and cathelicidin are the most frequently studied human AMPs. Our goals were to determine the distribution of AMPs and evaluate their mRNA expression in normal canine skin. Skin biopsies were taken from six healthy beagles. The relative transcript level of canine cathelicidin (cCath) and β-defensin (cBD)-1, cBD2 and cBD3 mRNA was quantified using quantitative real-time polymerase chain reaction. Indirect immunofluorescence (IIF), using polyclonal antibodies against cBD2, cBD3 and cCath, was used to evaluate protein localization in the skin of healthy dogs. The Pfaffl method, using experimentally determined primer efficiencies of amplification, was used to determine the expression level of cCath, cBD1 and cDB3 relative to cDB2. The levels of cCath, cBD3 and cBD1 mRNA were 358, 296 and 177 times higher than those of cBD2, respectively. Using IIF, cBD2 and cBD3 protein fluorescence was detected in all layers of the epidermis, whereas cCath was detected predominantly in the stratum granulosum and corneum. In addition, antimicrobial peptide detection was limited to the infundibular portion of the pilosebaceous units. We have v...Continue Reading

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Citations

Sep 6, 2011·Veterinary Immunology and Immunopathology·Domenico SantoroKaren L Campbell
Jan 22, 2013·Veterinary Dermatology·Domenico SantoroMariangela Segre
Jan 22, 2013·Veterinary Dermatology·Maryam Afshar, Richard L Gallo
Nov 13, 2013·Veterinary Dermatology·Domenico Santoro, Carol W Maddox
Jan 13, 2018·PloS One·Sheila M F TorresXiong Wang
Jul 10, 2019·Veterinary Dermatology·Domenico SantoroAntonio Di Loria
Nov 13, 2019·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Shelly AonoEdward E Morrison
Mar 14, 2012·Veterinary Dermatology·Brian C LeonardCharles L Bevins
May 26, 2021·Peptides·C E Valdez-MiramontesB Rivas-Santiago

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