Expression and distribution of cholesterol 7 alpha-hydroxylase in rat liver

Biochemical Pharmacology
P J BrassilD S Davies

Abstract

The hydroxylation of cholesterol by cholesterol 7 alpha-hydroxylase (CYP7) to 7 alpha-hydroxycholesterol is the rate-limiting step in the production of bile acids. An anti-peptide antibody targeted to the C-terminus of CYP7 was produced by immunising rabbits with the synthetic peptide Tyr-Lys-Leu-Lys-His. The antibody bound to a single band of 54 kDa from rat hepatic microsomal fractions. The intensity of the band was subject to a diurnal variation and showed a significant increase (P < 0.01) in apoprotein at night. Treatment of rats with cholestyramine increased CYP7 apoprotein in the morning (P < 0.005) and at night (P < 0.005), but diurnal variation was maintained. CYP7 catalytic activity, measured using a specific gas chromatography/mass spectrometry assay, showed similar changes in the pattern of diurnal variation and induction. The distribution of CYP7 in rat liver tissue sections was investigated by immunocytochemistry. In sections from rats treated with cholestyramine, there was an even distribution of immunoreactivity, except in the proximal perivenous hepatocytes where immunoreactivity was slightly more intense. A similar distribution was found in sections from untreated rat liver, except immunoreactivity was overall ...Continue Reading

References

Jun 15, 1992·Biochemical and Biophysical Research Communications·W G Karam, J Y Chiang
Jan 1, 1991·Methods in Enzymology·R J EdwardsA R Boobis
Jan 1, 1980·British Journal of Clinical Pharmacology·A R BoobisD S Davies
Apr 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W M PandakP B Hylemon

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Citations

Mar 9, 2005·Biochemical and Biophysical Research Communications·D Stroup, J R Ramsaran
Aug 11, 1998·Biochemical Pharmacology·P J BrassilR J Edwards
Apr 4, 1997·European Journal of Pharmacology·T Fujioka, Y Tsujita
Sep 14, 2013·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Surya B Prasad, Akalesh K Verma
Jan 7, 2014·Animal : an International Journal of Animal Bioscience·E ZoidisA C Pappas
Jul 3, 2002·The Journal of Clinical Investigation·Clive R PullingerJohn P Kane
Jan 5, 2000·Physiological Reviews·G J Schroepfer

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