Expression and distribution of cyclic GMP-dependent protein kinase-1 isoforms in human penile erectile tissue

The Journal of Sexual Medicine
Eginhard S WaldkirchPetter Hedlund

Abstract

Besides the bioavailability of nitric oxide (NO), downstream guanine monophosphate (cGMP) effector proteins are also considered to play a significant role in penile vascular disease. In animal studies, a downregulation of the cGMP-dependent protein kinase-1 (cGKI) alpha isoform has been linked to erectile dysfunction and diabetes mellitus. So far, the expression of cGKI alpha and beta isoforms has not been evaluated in human penile erectile tissue. To evaluate the expression of cGKI alpha and beta isoforms in relation to smooth muscle alpha-actin, cGMP, and endothelial NO synthase (eNOS) in human cavernous arteries (HCAs) and human corpus cavernosum (HCC). Cryostat sections of HCA and HCC were incubated with primary antibodies directed against alpha-actin, cGMP, eNOS, cGKI, cGKI alpha, and cGKI beta. Visualization of double-labeled immunofluorescent stainings was achieved by laser microscopy. Western blot analysis was performed in order to confirm the expression of cGKI isoforms. Expression of cGKI alpha and beta isoforms in relation to smooth muscle alpha-actin, cGMP, and eNOS in human penile erectile tissue. Immunoreactivities specific for cGKI, cGKI alpha, and cGKI beta were observed within the smooth musculature and the end...Continue Reading

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Citations

Oct 1, 2009·The Journal of Sexual Medicine·Roberta d'Emmanuele di Villa BiancaRaffaella Sorrentino
Nov 6, 2010·The Journal of Sexual Medicine·Francois GiulianoKim Wallen
Jan 14, 2009·The Journal of Sexual Medicine·Gerard P SergeantKeith D Thornbury
Jan 30, 2009·The Journal of Sexual Medicine·Shiu-Dong ChungJu-Ton Hsieh
Apr 14, 2017·International Journal of Impotence Research·S ÜckertP Hedlund
Jan 23, 2010·The Journal of Sexual Medicine·Christian GratzkeChristian G Stief
Mar 4, 2021·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Zhi S LiMichael D Gershon

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