Expression and distribution of cytochrome P450 enzymes in male rat kidney: effects of ethanol, acetone and dietary conditions

Biochemical Pharmacology
M J RonisT M Badger

Abstract

Ethanol, acetone, diet and starvation, known modulators of the hepatic cytochrome P450 (CYP)-dependent microsomal monooxygenase system, were assessed for their effects on cytochrome P450 isozyme content and monooxygenase activities in the male rat kidney. In acute experiments, rats were either treated with acetone, fasted or given a combination of the two treatments. Acetone treatment alone induced CYP2E1-dependent p-nitrophenol hydroxylase activity in kidney microsomes by 8-fold. This was accompanied by a 6-fold increase in CYP2E1 apoprotein as determined by Western blot analysis. There was, however, no significant increase in steady-state levels of CYP2E1 mRNA as measured by Northern blot analysis. Starvation also induced CYP2E1 apoprotein in the kidney and, as has been reported previously in the liver, had a synergistic inductive effect with acetone. CYP2B and CYP3A apoproteins were also induced by acetone, starvation and starvation/acetone combinations in the kidney. Immunohistochemical analysis revealed localization of CYP2E1 and CYP2B principally in the cortex associated with tubular cells. This distribution was maintained upon starvation/acetone treatment. Two induction experiments were performed in which the ethanol was...Continue Reading

References

Jan 1, 1991·Methods in Enzymology·C S YangJ S Yoo
Feb 15, 1992·European Journal of Biochemistry·R BühlerM Ingelman-Sundberg
Jun 1, 1991·European Journal of Biochemistry·M J RonisM Ingelman-Sundberg
Jan 1, 1990·Pharmacology & Therapeutics·C S Lieber
Sep 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D R Koop, D J Tierney
Dec 1, 1986·Archives of Biochemistry and Biophysics·C J PattenC S Yang
Nov 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·X MaC S Lieber

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Citations

Oct 29, 2000·Archives of Biochemistry and Biophysics·H M Peng, M J Coon
Jan 29, 2002·Archives of Biochemistry and Biophysics·D E FeiermanJ Zhang
Nov 13, 2004·The Journal of Nutritional Biochemistry·R K ReenT Moraes-Santos
Aug 6, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Myriam OrellanaRamón Rodrigo
Feb 27, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·B S CummingsL H Lash
Feb 21, 2006·Clinical and Experimental Pharmacology & Physiology·Christine CowplandKevin D Croft
Oct 29, 2005·BMC Clinical Pharmacology·Ina BergheimAlexandr Parlesak
Nov 7, 2002·Biological & Pharmaceutical Bulletin·Takahiro MukaiJunzo Nakamura
Apr 2, 2004·Biological & Pharmaceutical Bulletin·Takahiro MukaiJunzo Nakamura
Nov 7, 2009·The Journal of Veterinary Medical Science·Hyung-Sub KimMayumi Ishizuka
May 18, 2011·World Journal of Gastrointestinal Pharmacology and Therapeutics·Sanaa Ahmed AliMagda Mohamed Moustafa
Jan 1, 2008·Indian Journal of Clinical Biochemistry : IJCB·Subir Das Kumar, D M Vasudevan
Aug 4, 2009·Journal of Medical Entomology·Julia W PridgeonKenneth J Linthicum
Nov 5, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Sumaira SahreenRahmat Ali Khan
Jan 31, 2007·The Science of the Total Environment·Eric S JohnsonYu-Sheng Lin
Aug 9, 2003·The Journal of Pharmacy and Pharmacology·Barbora SzotákováVladimír Wsól
Feb 15, 2005·Journal of Ethnopharmacology·M OgeturkM Sarsilmaz
Feb 7, 2001·Biochemical Pharmacology·V LongoP G Gervasi
May 12, 2004·Chemico-biological Interactions·Guoyu LingXinxin Ding
May 25, 2015·Free Radical Biology & Medicine·Calivarathan LatchoumycandaneThomas M McIntyre
Jan 15, 2014·Free Radical Biology & Medicine·Calivarathan LatchoumycandaneThomas M McIntyre
Dec 3, 2014·Prostaglandins & Other Lipid Mediators·Ellen T McCarthyMukut Sharma
Oct 16, 2010·Acta histochemica·Ki Young KangSang Pil Yoon
Sep 24, 2004·Cell Biochemistry and Function·Murat OgeturkMustafa Sarsilmaz
Feb 1, 2007·Journal of Applied Toxicology : JAT·D AppenrothC Fleck
Apr 5, 2019·The Journal of Toxicological Sciences·Nana LongMin Dai
Feb 28, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Niwanthi W RajapakseRoger G Evans

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