Expression and distribution of genes encoding for polyamine-metabolizing enzymes in the different zones of male and female mouse kidneys

Amino Acids
O LevillainRafael Peñafiel

Abstract

The role of polyamines in renal physiology is only partially understood. Moreover, most of the data on the enzymes of polyamine metabolism come from studies using whole kidneys. The aim of the present study was to analyze the mRNA abundance of the genes implicated in both the polyamine biosynthetic and catabolic pathways in different renal zones of male and female mice, by means of the quantitative reverse transcription-polymerase chain reaction. Our results indicate that there is an uneven distribution of the different mRNAs studied in the five renal zones: superficial cortex, deep cortex, outer stripe of the outer medulla (OS), inner stripe of the outer medulla (IS), and the inner medulla + papilla (IM). The biosynthetic genes, ornithine decarboxylase (ODC) and spermine synthase, were more expressed in the cortex, whereas the mRNAs of the catabolic genes spermine oxidase (SMO) and diamine oxidase were more abundant in IS and IM. The genes involved in the regulation of polyamine synthesis (AZ1, AZ2 and AZIN1) were expressed in all the renal zones, predominantly in the cortex, while AZIN2 gene was more abundant in the OS. ODC, SMO, spermidine synthase and spermidine/spermine acetyl transferase expression was higher in males tha...Continue Reading

References

May 30, 1978·Biochemical and Biophysical Research Communications·H B BurchO H Lowry
Jan 1, 1979·Advances in Anatomy, Embryology, and Cell Biology·B Kaissling, W Kriz
Apr 1, 1978·Acta Physiologica Scandinavica·S HenningssonE Rosengren
Apr 22, 1992·Biochimica Et Biophysica Acta·M Manteuffel-CymborowskaB Grzelakowska-Sztabert
Jan 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·O A JänneL M Eisenberg
Apr 1, 1989·The American Journal of Physiology·J M SandsM A Knepper
Jul 31, 1986·Biochemical and Biophysical Research Communications·F G Berger, C W Porter
Sep 1, 1988·The Biochemical Journal·Y MurakamiS Hayashi
Aug 1, 1984·Kidney International·W G Guder, B D Ross
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K K KontulaO A Jänne
Jan 1, 1984·Cell Biochemistry and Function·A E Pegg
Jun 15, 1982·The Biochemical Journal·K FujitaS Hayashi
Jan 1, 1980·The International Journal of Biochemistry·W Pfaller, M Rittinger
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·T SuzukiK Igarashi
Jan 1, 1996·Trends in Biochemical Sciences·S HayashiS Matsufuji
Apr 30, 1998·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·H G SchwelbergerS D Kohlwein
Apr 27, 1999·The International Journal of Biochemistry & Cell Biology·L M Shantz, A E Pegg
Apr 18, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Z Zhou, Y J Kang
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·I P IvanovJ F Atkins
May 19, 2000·Amino Acids·O LevillainJ P Moulinoux
Sep 28, 2000·Pflügers Archiv : European journal of physiology·O LevillainD Soulet
Mar 27, 2001·Nature Reviews. Molecular Cell Biology·P Coffino
Apr 6, 2001·Cellular and Molecular Life Sciences : CMLS·T Thomas, T J Thomas
Apr 20, 2001·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·S BettuzziA Corti
Oct 6, 2001·Biochemical and Biophysical Research Communications·L T PitkänenL C Andersson
Jan 30, 2003·American Journal of Physiology. Renal Physiology·Kamyar ZahediManoocher Soleimani
Apr 24, 2003·American Journal of Physiology. Renal Physiology·Olivier LevillainMyriam Cayre
May 3, 2003·Biochemical and Biophysical Research Communications·Yanlin WangRobert A Casero

❮ Previous
Next ❯

Citations

Dec 21, 2012·Journal of the Science of Food and Agriculture·Irene Roncero-RamosMaría Pilar Navarro
Oct 12, 2018·Medical Sciences : Open Access Journal·Bruno Ramos-MolinaRafael Peñafiel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.