Expression and DNA methylation changes in human breast epithelial cells after bisphenol A exposure.

International Journal of Oncology
Sandra V FernandezJose Russo

Abstract

It has been suggested that xenoestrogens, a group of agents termed endocrine disruptors, may contribute to the development of hormone-dependent cancers, such as breast and endometrial cancers. We previously demonstrated that the xenoestrogen, bisphenol A (BPA), was able to induce the transformation in vitro of human breast epithelial cells. The normal-like human breast epithelial cell line, MCF-10F, formed tubules in collagen (3-D cultures), although after treatment with BPA (10-5 M and 10-6 M BPA) the cells produced less tubules (73% and 80%, respectively) and some spherical masses (27% and 20%, respectively). In the present study, expression and DNA methylation analyses were performed in these cells after exposure to BPA. These cells showed an increased expression of BRCA1, BRCA2, BARD1, CtIP, RAD51 and BRCC3, all of which are genes involved in DNA repair, as well as the downregulation of PDCD5 and BCL2L11 (BIM), both of which are involved in apoptosis. Furthermore, DNA methylation analysis showed that the BPA exposure induced the hypermethylation of BCL2L11, PARD6G, FOXP1 and SFRS11, as well as the hypomethylation of NUP98 and CtIP (RBBP8). Our results indicate that normal human breast epithelial cells exposed to BPA have in...Continue Reading

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Citations

Sep 26, 2013·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Pedro Martínez-PazGloria Morcillo
Mar 19, 2014·Environmental Toxicology and Pharmacology·Jaromir Michałowicz
Sep 11, 2014·International Journal of Molecular Sciences·Edna Ribeiro-VarandasMargarida Delgado
Jul 24, 2014·International Journal of Environmental Research and Public Health·Guergana MilevaCatherine Bielajew
Mar 21, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Nivedita ChatterjeeJinhee Choi
Dec 30, 2015·Toxicology and Applied Pharmacology·Manon C BouwmeesterLeo T M van der Ven
Mar 8, 2014·Cell Biology International·Yuka NishimuraNobuyuki Fukushima
Sep 3, 2013·Reproductive Toxicology·Johanna R Rochester
May 5, 2016·Molecular Nutrition & Food Research·Donato F RomagnoloOrnella I Selmin
Feb 10, 2017·Environmental and Molecular Mutagenesis·Natalie R Gassman
Jan 24, 2017·Molecular and Cellular Endocrinology·Danila CuomoConcetta Ambrosino
Mar 23, 2017·International Journal of Environmental Research and Public Health·Maddalena MallozziDonatella Caserta
Dec 2, 2017·Oncotarget·Bin LiuYuping Gao
May 3, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Lin HuiYihua Wu
May 30, 2020·International Journal of Molecular Sciences·Vidhya A NairWael M Abdel-Rahman
Apr 23, 2020·International Journal of Environmental Research and Public Health·Jin Hee Kim, Yun-Chul Hong
Mar 6, 2021·Environmental Science and Pollution Research International·Nadeem Ghani KhanShama Prasada Kabekkodu
May 26, 2018·Chemical Research in Toxicology·Manoj SonavaneNatalie R Gassman

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