Expression and DNA methylation of SERK , BBM , LEC2 and WUS genes in in vitro cultures of Boesenbergia rotunda (L.) Mansf

Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology
Rezaul KarimJennifer Ann Harikrishna

Abstract

The process of somatic embryogenesis and plant regeneration involve changes in gene expression and have been associated with changes in DNA methylation. Here, we report the expression and DNA methylation patterns of SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK), BABY BOOM (BBM), LEAFY COTYLEDON 2 (LEC2) and WUSCHEL (WUS) in meristematic block of newly emerged shoots from rhizome, embryogenic and non-embryogenic calli, prolonged cell suspension culture, ex vitro leaf, and in vitro leaf of regenerated plants of Boesenbergia rotunda. Among all seven samples, based on qRT-PCR, the highest level of expression of SERK, BBM and LEC2 was in embryogenic callus, while WUS was most highly expressed in meristematic block tissue followed by embryogenic callus. Relatively lower expression was observed in cell suspension culture and watery callus for SERK, LEC2 and WUS and in in vitro leaf for BBM. For gene specific methylation determined by bisulfite sequencing data, embryogenic callus samples had the lowest levels of DNA methylation at CG, CHG and CHH contexts of SERK, LEC2 and WUS. We observed negative correlation between DNA methylation at the CG and CHG contexts and the expression levels of SERK, BBM, LEC2 and WUS. Based on our resul...Continue Reading

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Citations

Apr 1, 2020·International Journal of Molecular Sciences·Barbara WójcikowskaMałgorzata D Gaj
May 17, 2019·Engineering in Life Sciences·Raul Sanchez-MuñozJavier Palazon
Oct 20, 2018·International Journal of Molecular Sciences·Pedro Osorio-MontalvoClelia De-la-Peña
Feb 13, 2020·Plant Molecular Biology·Renata Orłowska, Piotr Tomasz Bednarek
Jul 6, 2019·Frontiers in Plant Science·Taras Pasternak, Denes Dudits
Nov 16, 2021·Frontiers in Plant Science·Lucija MarkulinDunja Leljak Levanić

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Methods Mentioned

BETA
transgenic
dissecting
PCR
BS-seq

Software Mentioned

Methpipe
Methcounts
SPSS
Sengenics
Trim
Basic Local Alignment Search Tool
ExpressionSuite
BLASTx
Bismark

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