Expression and function of amphiregulin during murine preimplantation development

Molecular Reproduction and Development
E C TsarkL M Wiley

Abstract

Amphiregulin (Ar) is an EGF receptor ligand that functions to modulate the growth of both normal and malignant epithelial cells. We asked whether mouse preimplantation embryos express Ar, and if so, what the function of Ar is during preimplantation development. We used RT-PCR to show expression of Ar mRNA in mouse blastocysts, and using a polyclonal anti-Ar antibody and indirect immunofluorescence, we detected the presence of Ar protein in morula- and blastocyst-stage embryos. Ar protein was present in both the cytoplasm and nucleus in both morulae- and blastocyst-stage embryos, which is similar to Ar distribution in other cell types. Embryos cultured in Ar developed into blastocysts more quickly and also exhibited increased cell numbers compared to control embryos. In addition, 4-cell stage embryos cultured in an antisense Ar phosphorothioate-modified oligodeoxynucleotide (S-oligo) for 48 hr exhibited slower rates of blastocyst formation and reduced embryo cell numbers compared to embryos exposed to a random control S-oligo. TGF-alpha significantly improved blastocyst formation, but not cell numbers, for embryos cultured in the antisense Ar S-oligo. From these observations, we propose that Ar may function as an autocrine growt...Continue Reading

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Citations

Jan 5, 2000·Molecular Reproduction and Development·Z P YueM J Harper
Feb 9, 2002·Molecular Reproduction and Development·Jason St PierreShirley J Wright
Dec 8, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Kevin Y Lee, Francesco J DeMayo
Apr 26, 2008·Journal of Mammary Gland Biology and Neoplasia·Nicole E Willmarth, Stephen P Ethier
Oct 29, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S J JeongY H Suh
Mar 11, 2015·Veterinary Research Communications·Emel AlanHakan Sağsöz
Dec 26, 2001·Archives of Medical Research·L Díaz-Cueto, G L Gerton
Dec 31, 2003·Developmental Biology·Enrique Salas-Vidal, Hilda Lomelí
Jun 11, 2011·Biochimica Et Biophysica Acta·Benoit BusserAmandine Hurbin

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