Expression and function of CD300 in NK cells.

The Journal of Immunology : Official Journal of the American Association of Immunologists
Dikla LankryOfer Mandelboim

Abstract

The killing activity of NK cells is regulated by signals derived from inhibitory and activating NK cell receptors, including the CD300 family of proteins. CD300a was reported to be expressed on all NK cells and to deliver an inhibitory signal upon binding to a yet unknown ligand/s. The CD300a protein contains four ITIMs and is highly homologous to CD300c. Little is known about the function and distribution of these two receptors and the identity of their ligand/s. In this article, we show that CD300a is indeed an inhibitory receptor expressed by human NK cells, but surprisingly, we show that not all NK clones are inhibited in a CD300a-dependent manner. We demonstrate, using a panel of 13 new anti-CD300a and CD300c Abs that we generated, that CD300a and CD300c are indistinguishable on the surface of NK cells. Using mutational-analysis survey, we show that tyrosine 267 located in the third ITIM motif of the CD300a protein is important for the inhibitory function of CD300a.

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Citations

Jul 25, 2012·The Journal of Experimental Medicine·Chigusa Nakahashi-OdaAkira Shibuya
Nov 4, 2011·BMC Immunology·Venkateswara R SimhadriFrancisco Borrego
Mar 10, 2018·Nature Communications·Sai GeJun Qin
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May 17, 2013·European Journal of Immunology·Noa StanietskyOfer Mandelboim
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Oct 14, 2017·Scientific Reports·Ariella GlasnerOfer Mandelboim
Sep 16, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ariella GlasnerOfer Mandelboim
May 4, 2013·European Journal of Immunology·Dikla LankryOfer Mandelboim
May 17, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Olatz ZenarruzabeitiaFrancisco Borrego
Dec 2, 2020·Viruses·Naoto Koyanagi, Yasushi Kawaguchi
Feb 7, 2021·International Immunopharmacology·Yue CaoXiaohong Tian

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