Expression and function of integrin receptors for collagen and laminin in cultured human hepatic stellate cells

Gastroenterology
V CarloniP Gentilini

Abstract

Human hepatic stellate cells (HSCs), liver-specific pericytes, are currently considered major producers of extracellular matrix (ECM) components and key elements in the development of liver fibrogenesis. However, little is known about the possible functional interactions between HSCs and the various ECM components. Therefore, the present study was designed to evaluate the expression of integrins, the major family of extracellular matrix receptors. Integrin expression was evaluated by immunoprecipitation and confirmed by immunocytochemistry and flow cytometry. Human HSCs were shown to express alpha1beta1, alpha2beta1, alpha(v)beta1. Adhesion to type IV collagen, type I collagen, fibronectin, and laminin 1 was inhibited by anti-beta1 antibody identifying beta1-containing integrins as possible receptors for these components. In addition, we showed that HSCs express alpha6beta4, a heterodimer known to mediate adhesion of epithelial cells to laminin and not previously characterized in mesenchymal cells. Adhesion to laminin 1 was not inhibited by antibodies specific for alpha6 or beta4, thus establishing that laminin 1 is not a ligand for alpha6beta4 in this cell type. These findings represent the first description of integrin recept...Continue Reading

Citations

Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·I Rabinovitz, A M Mercurio
Sep 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H F Yee
Aug 10, 1999·Journal of Gastroenterology and Hepatology·D Li, S L Friedman
Jul 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·N ThéretB Clément
Aug 8, 2002·Archives of Histology and Cytology·Masahiro Amakawa, Yasuhisa Endo
Jul 8, 2003·The Journal of Pathology·Pierre Bedossa, Valérie Paradis
Jul 16, 2008·The Journal of Biological Chemistry·Katia Bourd-BoittinNathalie Théret
Nov 19, 2011·Journal of Medical Virology·Giovanni Faria SilvaMaria Inês de Moura Campos Pardini
Aug 17, 2000·Springer Seminars in Immunopathology·M Pinzani
Jul 19, 2008·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Sulochana Priya, Perumana R Sudhakaran
Mar 1, 2003·Pathology International·Mohammed S Razzaque, Takashi Taguchi
Mar 17, 2017·Anatomical Science International·Haruki SenooMutsunori Fujiwara
Apr 21, 1998·Liver·M PinzaniV Carloni
Aug 15, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Pierre BedossaMichel Vidaud
Oct 26, 2000·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M M de SouzaZ A Andrade
Jul 21, 1998·Journal of Pediatric Gastroenterology and Nutrition·R C Benyon, M J Arthur
Nov 12, 2010·Cell Biology International·Haruki SenooYoshihiro Mezaki
Jul 19, 2019·Biological Chemistry·Dieter Häussinger, Claus Kordes
May 19, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Milan DodigArthur J McCullough
Mar 27, 2004·The Journal of Biological Chemistry·Xiaoying ZhouR Christopher Benyon
Jul 19, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Tung-Yu TsuiSheung-Tat Fan
Aug 13, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Seung Ku LeeKyubum Kwack
Feb 2, 2012·Journal of Nutritional Science and Vitaminology·Noriko YamaguchiKiwamu Yoshikawa
Aug 30, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ralf JesnowskiMatthias Löhr
Jul 9, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Michitaka Matsuda, Ekihiro Seki
Dec 2, 1998·Journal of Hepatology·E Olaso, S L Friedman
May 10, 2002·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·T LamireauJ Rosenbaum
Jul 11, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Marianna D A GaçaR Christopher Benyon

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