Expression and function of K(v)7 channels in murine myometrium throughout oestrous cycle.

Pflügers Archiv : European journal of physiology
Laura A McCallumR M Tribe

Abstract

This study represents an extensive characterisation of the expression and functional impact of KCNQ and KCNE accessory subunits in a murine uterus using a combination of quantitative reverse transcription polymerase chain reaction, Western blot analysis, patch clamp electrophysiology and isometric tension recording. The use of uterine tissue throughout the oestrous cycle provided a physiological model with which to assess hormonal regulation of these genes. Messenger ribonucleic acid for all KCNQ genes were detected throughout the oestrous cycle with the KCNQ1 message predominant. KCNE isoforms were detected at each stage of the cycle. KCNE4 was the most abundant (p < 0.0001), and KCNQ1, KCNQ5 and KCNE1 were up-regulated in metestrous (p < 0.0001). The K(v)7 channel inhibitor XE991 reduced outward K(+) currents and significantly increased spontaneous myometrial contractions (p < 0.05), whereas retigabine (K(v)7 activator) significantly relaxed uterine tissues (p < 0.001). These data are the first to characterise KCNQ and KCNE gene expression in a cell type outside of neurons and the cardiovascular system.

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Citations

Apr 25, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Thomas A JeppsSusumu Ohya
Jul 12, 2011·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·V IpavecD Currò
Jun 10, 2014·British Journal of Pharmacology·T A JeppsI A Greenwood
Oct 15, 2013·Experimental Physiology·Iain A Greenwood, Rachel M Tribe
Oct 15, 2013·European Journal of Pharmacology·Alice AdduciDiego Currò
Apr 15, 2014·European Journal of Pharmacology·Diego Currò
Jul 13, 2011·Circulation·Thomas A JeppsIain A Greenwood
Sep 8, 2011·Basic & Clinical Pharmacology & Toxicology·Julie SvaløFrederik Rode
Oct 2, 2019·Proceedings of the National Academy of Sciences of the United States of America·Rían W ManvilleGeoffrey W Abbott
May 16, 2009·The Journal of Physiology·Kenneth L Byron, Lioubov I Brueggemann
Jul 23, 2020·Frontiers in Physiology·Jennifer van der HorstThomas A Jepps
Feb 6, 2010·Journal of Cellular and Molecular Medicine·Laura A McCallumRachel M Tribe
Jul 14, 2010·The Journal of Physiology·Xi Zoë ZhongIain A Greenwood
Oct 28, 2015·The Journal of Physiology·Thomas A JeppsIain A Greenwood
Oct 11, 2017·Annual Review of Pharmacology and Toxicology·Vincenzo BarreseIain A Greenwood
Oct 13, 2020·Frontiers in Physiology·John Malysz, Georgi V Petkov
Nov 8, 2020·Annual Review of Physiology·Susan Wray, Sarah Arrowsmith
May 29, 2021·RSC Medicinal Chemistry·Matteo BorginiPeter Wipf

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