Expression and function of microRNA-9 in the mid-hindbrain area of embryonic chick

BMC Developmental Biology
A Alwin Prem AnandAndrea Wizenmann

Abstract

MiR-9 is a small non-coding RNA that is highly conserved between species and primarily expressed in the central nervous system (CNS). It is known to influence proliferation and neuronal differentiation in the brain and spinal cord of different vertebrates. Different studies have pointed to regional and species-specific differences in the response of neural progenitors to miR-9. In ovo and ex ovo electroporation was used to overexpress or reduce miR-9 followed by mRNA in situ hybridisation and immunofluorescent stainings to evaluate miR- expression and the effect of changed miR-9 expression. We have investigated the expression and function of miR-9 during early development of the mid-hindbrain region (MH) in chick. Our analysis reveals a closer relationship of chick miR-9 to mammalian miR-9 than to fish and a dynamic expression pattern in the chick neural tube. Early in development, miR-9 is diffusely expressed in the entire brain, bar the forebrain, and it becomes more restricted to specific areas of the CNS at later stages. MiR-9 overexpression at HH9-10 results in a reduction of FGF8 expression and premature neuronal differentiation in the mid-hindbrain boundary (MHB). Within the midbrain miR-9 does not cause premature neuron...Continue Reading

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Citations

Dec 7, 2018·PloS One·Lucia Natalia MoroSantiago Gabriel Miriuka
Dec 18, 2020·Frontiers in Cell and Developmental Biology·A Alwin Prem AnandAndrea Wizenmann
Jul 6, 2019·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Arubala P ReddyNurgul Carkaci-Salli
Sep 15, 2021·Technology in Cancer Research & Treatment·Cheng-You JiaDa Fu

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Methods Mentioned

BETA
antisense oligonucleotides
antisense oligonucleotide
transfection
confocal microscopy
transfections
transfect

Software Mentioned

Axiovision
miRbase
Amira
MEGA
RNALogo
ZEN
MUSCLE

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