Expression and function of transmembrane 4 superfamily proteins in digestive system cancers

Cancer Cell International
Yaoyue QiWensheng Qiu

Abstract

Although the medical level is constantly improving, cancer is still a major disease that threatens human health, and very effective treatments have not been found. In recent years, studies have found that four-transmembrane superfamily proteins are involved in multiple stages of tumorigenesis and development, but their expression and function in tumors have not been systematically studied. We used the Oncomine database to analyze the mRNA expression levels of TSPAN family in various cancers. Then differentially expressed genes were screened out and verified by liver cancer, colorectal cancer, and gastric cancer cells by q-PCR and Western blot analysis. CCK8 and EDU analysis are used to detect cell proliferation, Cell wound scrape assay and Cell invasion assay are used to analyze cell invasion and metastasis. Nude tumor formation test used to verify the tumor suppressive effect of TSPAN7 in vivo. Differential analysis of 33 TSPAN proteins revealed that a total of 11 proteins showed differential expression in 10% of independent analyses, namely TSPAN1, TSPAN3, TSPAN5, TSPAN6, TSPAN7, TSPAN8, TSPAN13, TSPAN25, TSPAN26, TSPAN29, TSPAN30. TSPAN7 is the only four-transmembrane protein with reduced expression in three types of digesti...Continue Reading

References

Apr 8, 2004·Neoplasia : an International Journal for Oncology Research·Daniel R RhodesArul M Chinnaiyan
Feb 24, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Simone Diermeier-DaucherGero Brockhoff
Apr 25, 2009·International Journal of Cancer. Journal International Du Cancer·Daniela WuttigManfred P Wirth
Jul 13, 2011·Methods in Molecular Biology·Giles Cory
Sep 17, 2013·Methods in Enzymology·Julia Bachman
Feb 8, 2014·Nature Reviews. Cancer·Martin E Hemler
May 27, 2014·Cancer Letters·Antonio MazzoccaVinicio Carloni
Jun 26, 2014·Journal of Visualized Experiments : JoVE·Calvin R JustusLi V Yang
Aug 17, 2014·Journal of Cell Science·Stéphanie CharrinEric Rubinstein
Mar 26, 2015·World Journal of Gastroenterology : WJG·Yoko MurayamaShusaku Tsutsui
Apr 9, 2015·Frontiers in Physiology·Kyle A BeckwithNatarajan Muthusamy
Jun 20, 2015·Cell Adhesion & Migration·Xupin JiangYuesheng Huang
Dec 21, 2016·Cancer Biotherapy & Radiopharmaceuticals·Zheng DongZhiguang Geng
Apr 15, 2017·Biochemical Society Transactions·Ibrahim H YaseenLynda J Partridge
Apr 22, 2017·Frontiers in Cell and Developmental Biology·Christina M Termini, Jennifer M Gillette
May 10, 2017·Cancer Letters·Wanqing ChenJie He
May 16, 2017·Methods in Molecular Biology·Brianna Kim
Jul 4, 2017·Scandinavian Journal of Public Health·Miranda M FidlerIsabelle Soerjomataram
Aug 16, 2017·In Vitro Cellular & Developmental Biology. Animal·John M BaustFrank Simione
Sep 21, 2017·Oncology Letters·Yaowu CaiXuefeng Xu
Jun 12, 2018·Frontiers in Immunology·Luise Florin, Thorsten Lang
Aug 4, 2018·Frontiers in Immunology·Louisa YeungMark D Wright
Sep 24, 2018·Methods in Molecular Biology·Shuang Liu
Dec 6, 2018·Journal of Experimental & Clinical Cancer Research : CR·Yan WangLianxin Liu
Feb 1, 2019·Journal of Cellular and Molecular Medicine·Ya-Wen WangRong Ma
Aug 8, 2019·Proteomics·Nazha Sidahmed-AdrarFrançois Le Naour

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
protein assay
electrophoresis
xenograft
transfection
xenografts

Software Mentioned

Oncomine
SPSS
SPSS11

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.