Expression and functional activity of PPARgamma in pancreatic beta cells

British Journal of Pharmacology
Hannah J WeltersNoel G Morgan

Abstract

Rosiglitazone is an agonist of peroxisome proliferator activated receptor-gamma (PPARgamma) and ameliorates insulin resistance in type II diabetes. In addition, it may also promote increased pancreatic beta-cell viability, although it is not known whether this effect is mediated by a direct action on the beta cell. We have investigated this possibility. Semiquantitative real-time reverse transcription-polymerase chain reaction analysis (Taqman) revealed that freshly isolated rat islets and the clonal beta-cell line, BRIN-BD11, express PPARgamma, as well as PPARalpha and PPARdelta. The levels of expression of PPARgamma were estimated by reference to adipose tissue and were found to represent approximately 60% (islets) and 30% (BRIN-BD11) of that found in freshly isolated visceral adipose tissue. Western blotting confirmed the presence of immunoreactive PPARgamma in rat (and human) islets and in BRIN-BD11 cells. Transfection of BRIN-BD11 cells with a PPARgamma-sensitive luciferase reporter construct was used to evaluate the functional competence of the endogenous PPARgamma. Luciferase activity was modestly increased by the putative endogenous ligand, 15-deoxy-Delta12,14 prostaglandin J2 (15dPGJ2). Rosiglitazone also caused activa...Continue Reading

References

Mar 7, 1998·The Journal of Biological Chemistry·M ShimabukuroR H Unger
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y T ZhouR H Unger
Jan 26, 1999·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·H IshidaY Seino
Aug 23, 2000·The Journal of Clinical Investigation·J M Olefsky
Apr 13, 2001·European Journal of Pharmacology·G FerryJ A Boutin
Mar 14, 2002·Diabetes Research and Clinical Practice·Toshihide KawaiTakao Saruta
Apr 17, 2002·Cellular Signalling·Olivier Nosjean, Jean A Boutin
Apr 19, 2002·Biochemical Pharmacology·Miriam CnopDaniel G Pipeleers
Jul 30, 2002·Diabetes·Michael Stumvoll, Hans Häring
Sep 20, 2002·Molecular & Cellular Proteomics : MCP·Jean-Charles SanchezMichael A Cawthorne
Sep 21, 2002·Diabetologia·C Fürnsinn, W Waldhäusl
Dec 10, 2002·Biochemical and Biophysical Research Communications·Katrin EitelMonika Kellerer
Jan 22, 2003·Biochemical and Biophysical Research Communications·Buckminster Farrow, B Mark Evers
Feb 22, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Padma Kumar NarayananPeter Bugelski
Feb 28, 2003·Expert Opinion on Investigational Drugs·M Tadayyon, S A Smith
Mar 22, 2003·The Journal of Pharmacology and Experimental Therapeutics·Stefano LauroraGiuseppina Barrera
Jun 25, 2003·Biochemical and Biophysical Research Communications·Yoko NakamichiShinya Nagamatsu
Jun 27, 2003·Drugs·Michaela Diamant, Robert J Heine
Jul 2, 2003·Expert Opinion on Investigational Drugs·Michael Stumvoll

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Citations

Feb 14, 2009·Pancreas·Caroline Fernandes-SantosCarlos Alberto Mandarim-de-Lacerda
Sep 14, 2013·Hepatitis Monthly·Mandana Mahmoodzadeh SaghebRamin Yaghobi
Jun 20, 2013·Mediators of Inflammation·Francisco A MonsalveRodrigo Moore-Carrasco
Jan 15, 2008·Biochemical and Biophysical Research Communications·Hyo-Sup KimMoon-Kyu Lee
Apr 16, 2015·Mediators of Inflammation·Luciane Alarcão Dias-MelicioAngela Maria Victoriano Campos Soares
Aug 3, 2016·International Journal of Molecular Sciences·Attila PapLaszlo Nagy
Sep 14, 2006·American Journal of Physiology. Endocrinology and Metabolism·Neda RasouliSteven C Elbein
Oct 2, 2012·Molecular Pharmacology·Martina DüferGisela Drews
Nov 30, 2006·Clinical Science·Philip NewsholmeNoel G Morgan
Apr 28, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·C Y IvashchenkoR M Mortensen
Jan 15, 2011·British Journal of Pharmacology·N SzentandrássyP P Nánási
Jun 17, 2021·Journal of Cellular Physiology·Mina HwangMoon-Kyu Lee

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