Expression and functional analysis of Na(+)-dependent glutamate transporters from zebrafish brain

Brain Research Bulletin
Eduardo Pacheco RicoMaurício Reis Bogo

Abstract

High-affinity excitatory amino acid transporters (EAATs) regulate extracellular glutamate levels. Zebrafish (Danio rerio) provides an excellent model to study the function of different neurotransmitter systems. Although the identification of the EAAT family is well established in the mammalian central nervous system (CNS), EAAT-related genes and their expression profile in zebrafish have not yet been reported. Here we identify and describe the expression profile of EAATs-related genes and functional properties of glutamate uptake in three major brain structures from zebrafish (telencephalon, optic tectum and cerebellum). Searches on zebrafish genome databases and a phylogenetic analysis confirmed the presence of several EAAT-related genes (EAAT2, EAAT3, three EAAT1 paralogs and two EAAT5 sequences). All sequences identified were expressed in the structures analyzed. EAAT2 and EAAT3 were the most prominent glutamate transporters expressed in all brain areas. A uniform expression was observed for EAAT1A, whereas higher EAAT1B transcript levels were detected in telencephalon. Lower amounts of EAAT1C transcripts were observed in cerebellum when compared to other structures. No EAAT4-related sequence was found in the zebrafish genom...Continue Reading

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Citations

Nov 26, 2014·Molecular Neurobiology·Fernanda Cenci VuadenAngela T S Wyse
Sep 13, 2011·Neurotoxicology and Teratology·E P RicoC D Bonan
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Mar 10, 2021·Neurotoxicity Research·Baban S Thawkar, Ginpreet Kaur
Jun 25, 2021·Analytical and Bioanalytical Chemistry·J MarfàM I Pividori
Feb 28, 2020·Neurochemistry International·Francini FranscesconDenis B Rosemberg

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