PMID: 11316324Apr 24, 2001Paper

Expression and functional characterization of the mt1 melatonin receptor from rat brain in Xenopus oocytes: evidence for coupling to the phosphoinositol pathway

Journal of Pineal Research
C BlumenauU Musshoff

Abstract

Melatonin-sensitive receptors were expressed in Xenopus laevis oocytes following an injection of mRNA from rat brain. The administration of 0.1-100 micromol/L melatonin to voltage-clamped oocytes activates calcium-dependent chloride currents via a pertussis toxin-sensitive G protein and the phosphoinositol pathway. To determine which melatonin receptor type (mt1, MT2, MT3) is functionally expressed in the Xenopus oocytes, we used (i) agonists and antagonists of different receptor types to characterize the pharmacological profile of the expressed receptors and (ii) a strategy of inhibiting melatonin receptor function by antisense oligonucleotides. During pharmacological screening administration of the agonists 2-iodomelatonin and 2-iodo-N-butanoyl-5-methoxytryptamine (IbMT) to the oocytes resulted in oscillatory membrane currents, whereas the administration of the MT3 agonist 5-methoxycarbonylamino-N-acetyltryptamine (GR135,531) exerted no detectable membrane currents. The melatonin response was abolished by a preceding administration of the antagonists 2-phenylmelatonin and luzindole but was unaffected by the MT3 antagonist prazosin and the MT2 antagonist 4-phenyl-2-propionamidotetralin (4-P-PDOT). In the antisense experiments,...Continue Reading

Citations

May 10, 2002·Hippocampus·Ulrich MusshoffErwin-Josef Speckmann
Jan 26, 2013·Pharmacology & Therapeutics·Laurence LanfumeyMichel Hamon
Sep 10, 2005·Journal of Pineal Research·Andreas G BachElmar Peschke
Jan 31, 2006·Journal of Pineal Research·Yung-Wui TjongMan-Lung Fung
Oct 4, 2006·Journal of Pineal Research·Michael J JarzynkaPaula A Witt-Enderby
Apr 24, 2015·Journal of Pineal Research·Elmar PeschkeEckhard Mühlbauer
Mar 29, 2013·International Journal of Molecular Sciences·Elmar PeschkeEckhard Mühlbauer
Aug 8, 2006·The Journal of Veterinary Medical Science·Masaru UsuiKoichi Mamba
Mar 29, 2003·Journal of Pineal Research·Andrea Vieira SouzaAna Maria de Lauro Castrucci

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.