Abstract
The Id family of helix-loop-helix factors (Id1, Id2, and Id3) expressed in many types of cells has been reported to negatively regulate myoblast differentiation and is required for G1/S progression of arrested fibroblasts. Our previous studies have indicated that Id1, Id2, and Id3 mRNA expression appear in the subventricular zone of 1-day-old rat brains. At later ages, Id3 mRNA was only expressed in astrocytes. We now report that Id1 and Id3 mRNA expression increased in astrocytes during the first hour of serum stimulation. Subsequently, the Id1 and Id3 mRNA levels gradually declined to basal level as observed in cultures without serum stimulation. However, there was no significant difference in Id2 mRNA expression between serum-treated and control astrocyte cultures within 1 h of serum induction. In addition, a strong nuclear immunostaining for Id2 and Id3 proteins was observed 24 h after serum stimulation. This observation is consistent with our results that show an increase in Id2 and Id3 protein levels following 24 h serum induction. Furthermore, DNA synthesis in FCS-stimulated astrocytes was blocked by antisense oligonucleotides against Id3 mRNA. The addition of Id3 antisense oligonucleotides caused approximately 50% reduc...Continue Reading
References
Mar 27, 1992·Science·B L KreiderT Kadesch
Nov 1, 1992·Developmental Biology·M DuncanK Chada
Jan 1, 1992·Journal of Neuroscience Research·J C LouisS Varon
Aug 1, 1992·Genes & Development·Y JenR Benezra
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J BiggsM A Israel
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B A ChristyD Nathans
Apr 6, 1990·Cell·R BenezraH Weintraub
Jul 1, 1989·Journal of Neuroscience Research·A T ArenanderH R Herschman
Jul 1, 1989·Journal of Neuroscience Research·A T ArenanderH R Herschman
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K UnsickerM Sensenbrenner
Jun 1, 1980·The Journal of Cell Biology·K D McCarthy, J de Vellis
Jan 1, 1983·Journal of Neuroscience Research·J A SeversonC E Finch
Sep 24, 1993·Cell·L A Neuhold, B Wold
Jun 1, 1994·Genes & Development·A IavaroneM A Israel
Mar 11, 1994·Nucleic Acids Research·V RiechmannF Sablitzky
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·M V BaroneL Philipson
Nov 1, 1993·Developmental Biology·T NeumanH O Nornes
Jul 7, 1997·Neuroreport·S P Persengiev, D L Kilpatrick
Mar 25, 2000·Endocrine-related Cancer·C Q LinP Y Desprez
Jan 1, 1985·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M WeibelM Sensenbrenner
Citations
Dec 18, 2001·Journal of Neuroscience Research·S F TzengJ de Vellis
Jan 9, 2009·Cellular and Molecular Neurobiology·Shinya ObayashiJun-ichi Satoh
Oct 6, 2000·Neuroscience Letters·J P WuS F Tzeng
Feb 2, 1999·Neuroreport·P J Andres-BarquinM A Israel
Aug 10, 2001·Neuroreport·E AronicaJ A Gorter
Nov 22, 2005·Acta Pharmacologica Sinica·Robert Wai-Sui Lim, Jin-Mei Wu
Nov 22, 2007·Neuroscience Letters·Xing-Shu ChenZhong-Xiang Yao
Aug 24, 2005·Histopathology·W C XueA N Y Cheung
Sep 21, 2004·Journal of Cellular Biochemistry·Joon-Ik AhnYong-Sung Lee
Jul 11, 2013·Glia·Anna V MolofskyMichael C Oldham
May 19, 2006·The European Journal of Neuroscience·Anders I PerssonPeter S Eriksson
Apr 13, 2001·Neuron·S WangB A Barres
Mar 6, 1999·Experimental Cell Research·P J Andres-BarquinM A Israel
May 15, 2002·Glia·Dmitri A A VandeputteEleonora Aronica
Jun 29, 1999·Glia·S F TzengJ De Vellis
Nov 14, 1998·Glia·S F Tzeng, J de Vellis
Jul 28, 2020·Cells·Shang-Der ChenDing-I Yang
Mar 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jean-Philippe CoppePierre-Yves Desprez