PMID: 9191087Jun 1, 1997Paper

Expression and functional role of the Id HLH family in cultured astrocytes

Brain Research. Molecular Brain Research
S F Tzeng, J de Vellis

Abstract

The Id family of helix-loop-helix factors (Id1, Id2, and Id3) expressed in many types of cells has been reported to negatively regulate myoblast differentiation and is required for G1/S progression of arrested fibroblasts. Our previous studies have indicated that Id1, Id2, and Id3 mRNA expression appear in the subventricular zone of 1-day-old rat brains. At later ages, Id3 mRNA was only expressed in astrocytes. We now report that Id1 and Id3 mRNA expression increased in astrocytes during the first hour of serum stimulation. Subsequently, the Id1 and Id3 mRNA levels gradually declined to basal level as observed in cultures without serum stimulation. However, there was no significant difference in Id2 mRNA expression between serum-treated and control astrocyte cultures within 1 h of serum induction. In addition, a strong nuclear immunostaining for Id2 and Id3 proteins was observed 24 h after serum stimulation. This observation is consistent with our results that show an increase in Id2 and Id3 protein levels following 24 h serum induction. Furthermore, DNA synthesis in FCS-stimulated astrocytes was blocked by antisense oligonucleotides against Id3 mRNA. The addition of Id3 antisense oligonucleotides caused approximately 50% reduc...Continue Reading

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Citations

Jan 9, 2009·Cellular and Molecular Neurobiology·Shinya ObayashiJun-ichi Satoh
May 19, 2006·The European Journal of Neuroscience·Anders I PerssonPeter S Eriksson
Mar 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jean-Philippe CoppePierre-Yves Desprez

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