Expression and functional roles of estrogen receptor GPR30 in human intervertebral disc

The Journal of Steroid Biochemistry and Molecular Biology
Aiqun WeiAshish D Diwan

Abstract

Estrogen withdrawal, a characteristic of female aging, is associated with age-related intervertebral disc (IVD) degeneration. The function of estrogen is mediated by two classic nuclear receptors, estrogen receptor (ER)-α and -β, and a membrane bound G-protein-coupled receptor 30 (GPR30). To date, the expression and function of GPR30 in human spine is poorly understood. This study aimed to evaluate GPR30 expression in IVD, and its role in estrogen-related regulation of proliferation and apoptosis of disc nucleus pulposus (NP) cells. GPR30 expression was examined in 30 human adult NP and 9 fetal IVD. Results showed that GPR30 was expressed in NP cells at both mRNA and protein levels. In human fetal IVD, GPR30 protein was expressed in the NP at 12-14 weeks gestation, but was undetectable at 8-11 weeks. The effect of 17β-estradiol (E2) on GPR30-mediated proliferation and interleukin-1β (IL-1β)-induced apoptosis of NP cells was investigated. Cultured NP cells were treated with or without E2, GPR30 antagonist G36, and ER antagonist ICI 182,780. NP cell viability was tested by MTS assay. Apoptosis was determined by flow cytometry using fluorescence labeled annexin-V, TUNEL assay and immumnocytochemical staining of activated caspase-3...Continue Reading

References

May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D SömjenA M Kaye
Sep 12, 2003·Trends in Pharmacological Sciences·Jan-Ake Gustafsson
Nov 1, 2003·The Spine Journal : Official Journal of the North American Spine Society·Molly T VogtJane A Cauley
Feb 12, 2005·Science·Chetana M RevankarEric R Prossnitz
Aug 23, 2005·Human Reproduction·Yves Muscat BaronNeville Calleja
Aug 30, 2005·Trends in Endocrinology and Metabolism : TEM·Edward J Filardo, Peter Thomas
Mar 8, 2006·Nature Chemical Biology·Cristian G BologaEric R Prossnitz
Apr 6, 2006·The Journal of Bone and Joint Surgery. American Volume·Sally RobertsJanis Menage
Jun 20, 2006·Biochemical and Biophysical Research Communications·Takeshi FunakoshiYoichi Mizukami
Jul 7, 2007·Physiological Reviews·Nina HeldringJan-Ake Gustafsson
Sep 20, 2007·The Journal of Clinical Endocrinology and Metabolism·Andrei S Chagin, Lars Sävendahl
Oct 4, 2007·Apoptosis : an International Journal on Programmed Cell Death·Chang-Qing ZhaoLi-Yang Dai
Feb 15, 2008·Annual Review of Physiology·Eric R ProssnitzHelen J Hathaway
Apr 25, 2008·The Journal of Endocrinology·Terhi J HeinoLars Sävendahl
May 12, 2009·Nature Chemical Biology·Megan K DennisEric R Prossnitz
Sep 3, 2009·Menopause International·J Calleja-AgiusM P Brincat
Sep 8, 2009·Trends in Endocrinology and Metabolism : TEM·Björn Olde, L M Fredrik Leeb-Lundberg
Aug 25, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jeffery FordOrhan K Oz
Oct 21, 2010·Radiology·Yi-Xiang J Wang, James F Griffith
Feb 2, 2013·The Spine Journal : Official Journal of the North American Spine Society·Nam V VoJames D Kang
Mar 21, 2013·Apoptosis : an International Journal on Programmed Cell Death·Fan DingLi-ming Xiong
Jan 18, 2014·Apoptosis : an International Journal on Programmed Cell Death·Si-Dong YangYan-Li Song
Jul 16, 2014·Climacteric : the Journal of the International Menopause Society·C LouH-Z Xu
Dec 20, 2014·Cell Death and Differentiation·S ShaliniS Kumar

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Citations

Feb 2, 2018·Digestive Diseases and Sciences·Xubiao NieBiguang Tuo
Jun 14, 2017·Menopause : the Journal of the North American Menopause Society·Chao LouDengwei He
Jun 1, 2017·Canine Genetics and Epidemiology·Janelle M BelangerAnita M Oberbauer
Nov 2, 2019·Ageing Research Reviews·Sidong YangWenyuan Ding
Dec 10, 2019·Steroids·Lin-Yu JinXin-Feng Li

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