Expression and Functional Roles of the Pepper Pathogen-Induced bZIP Transcription Factor CabZIP2 in Enhanced Disease Resistance to Bacterial Pathogen Infection

Molecular Plant-microbe Interactions : MPMI
Chae Woo LimSung Chul Lee

Abstract

A pepper bZIP transcription factor gene, CabZIP2, was isolated from pepper leaves infected with a virulent strain of Xanthomonas campestris pv. vesicatoria. Transient expression analysis of the CabZIP2-GFP fusion protein in Nicotiana benthamiana revealed that the CabZIP2 protein is localized in the cytoplasm as well as the nucleus. The acidic domain in the N-terminal region of CabZIP2 that is fused to the GAL4 DNA-binding domain is required to activate the transcription of reporter genes in yeast. Transcription of CabZIP2 is induced in pepper plants inoculated with virulent or avirulent strains of X. campestris pv. vesicatoria. The CabZIP2 gene is also induced by defense-related hormones such as salicylic acid, methyl jasmonate, and ethylene. To elucidate the in vivo function of the CabZIP2 gene in plant defense, virus-induced gene silencing in pepper and overexpression in Arabidopsis were used. CabZIP2-silenced pepper plants were susceptible to infection by the virulent strain of X. campestris pv. vesicatoria, which was accompanied by reduced expression of defense-related genes such as CaBPR1 and CaAMP1. CabZIP2 overexpression in transgenic Arabidopsis plants conferred enhanced resistance to Pseudomonas syringae pv. tomato DC3...Continue Reading

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Citations

Dec 15, 2018·The Plant Journal : for Cell and Molecular Biology·Hyunhee JooSung Chul Lee
Sep 8, 2017·Biotechnology Letters·Ali NomanShuilin He
Jan 5, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Lijuan ZhouHenry T Nguyen
Jan 6, 2021·Molecular Plant-microbe Interactions : MPMI·Matthew J PriorWolf B Frommer
Apr 15, 2021·Molecular Plant-microbe Interactions : MPMI
Jun 1, 2021·Genome Génome / Conseil National De Recherches Canada·Xiaojuan ZhongQiantao Jiang

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