Expression and functional significance of an activation-dependent epitope of the beta 1 integrins in chronic inflammatory diseases

European Journal of Immunology
A G ArroyoF Sánchez-Madrid

Abstract

The avidity of VLA integrins for their ligands can be increased by their transition to an active conformational state. This conformational change can be detected with a novel monoclonal antibody (mAb), termed 15/7, that recognizes an activation-dependent conformational epitope on the common beta 1 polypeptide of different VLA alpha beta 1 integrins. In an attempt to understand the possible role of the active conformational state of beta 1 integrins in vivo, we first investigated the expression of 15/7 epitope on T lymphocytes from patients with chronic inflammatory joint diseases. An enhanced expression of the 15/7 epitope was found in the synovial fluid (SF) T lymphocytes from these patients as compared to their peripheral blood (PB) T cells. The effect of different cytokines on the appearance of the 15/7 activation epitope in PB T lymphocytes was subsequently analyzed; interferon-gamma, interleukin-2 and, to a lower extent, tumor necrosis factor-alpha were able to induce an increased expression of the 15/7 epitope. This enhanced 15/7 expression correlated with a higher binding ability to fibronectin of cytokine-activated T cells. The presence of this activation epitope was detected in a small proportion of T lymphocytes scatt...Continue Reading

References

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Citations

May 26, 1998·Journal of Neuroimmunology·B EngelhardtS A Michie
Oct 9, 1998·The Journal of Investigative Dermatology·M BoukhelifaF Braut-Boucher
Feb 12, 1998·The Journal of Biological Chemistry·G Chi-RossoV E Koteliansky
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Nov 1, 1995·Clinical and Diagnostic Laboratory Immunology·A Gorski, J W Kupiec-Weglinski

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