Expression and immunogenicity of an Echinococcus granulosus fatty acid-binding protein in live attenuated Salmonella vaccine strains.

Infection and Immunity
José ChabalgoityC E Hormaeche

Abstract

Fatty acid-binding proteins (FABPs) are candidate molecules for vaccines against several parasitic platyhelminths. A FABP from the cestode Echinococcus granulosus (EgDf1) was expressed in Salmonella vaccine strains as a C-terminal fusion to fragment C of tetanus toxin (TetC) by using expression vector pTECH. The fusion protein was equally expressed in several attenuated vaccine strains derived from bacteria with different genetic backgrounds and different attenuating mutations. Single-dose immunization experiments with the aroA Salmonella typhimurium strain SL3261 carrying the pTECH-EgDf1 construct were conducted with mice, using both the intravenous and the oral routes. Surprisingly, the antibody response to EgDf1 and the antigen-specific cytokine production in spleen cells were stronger in mice immunized orally. Furthermore, immune mouse sera strongly reacted with fixed sections of the worm's larval stage. Analysis of the isotype distribution of the specific anti-EgDf1 antibodies showed similar production of immunoglobulin G1 (IgG1) and IgG2a together with specific IgA antibodies. In addition, stimulation of spleen cells from mice immunized with the different constructs with either Salmonella lysate, TetC, or EgDf1 showed tha...Continue Reading

References

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Citations

Nov 5, 2002·FEMS Microbiology Reviews·Helen S GarmoryRichard W Titball
Dec 3, 1999·Immunological Reviews·R M MaizelsM Yazdanbakhsh
Apr 13, 2005·Parasitology Research·M FraizeA F Petavy
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Mar 13, 2001·The Veterinary Journal·P MastroeniG Dougan
Jun 24, 2003·Biochimica Et Biophysica Acta·Emma JakobssonGerard J Kleywegt

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