Expression and induction of the stress protein alpha-B-crystallin in vascular endothelial cells

Histochemistry and Cell Biology
Nikola GolenhofenDetlev Drenckhahn

Abstract

Stress-induced development of enhanced tolerance against various kinds of stresses has been observed in vascular endothelial cells as well as in several other cell types. Stress proteins are thought to play a key role in the development of stress tolerance. In this study we show that endothelial cells of various sources contain the major stress protein of the eye lens, alphaB-crystallin. In the mouse myocardial microvascular cell line, MyEnd, alphaB-crystallin as well as the heat shock proteins HSP 70i and HSP 25 display a low constitutive expression but can be significantly upregulated by sodium arsenite stress. Osmotic stress also resulted in strong upregulation of alphaB-crystallin and HSP 70i but not of HSP 25. Both osmotic and arsenite stress resulted in significant stress tolerance of MyEnd cells against glucose deprivation as assayed by lactate dehydrogenase release and overall cellular morphology. Development of stress tolerance without induction of HSP 25 indicates that HSP 25 is not essential for the protective effect. MyEnd cells from alphaB-crystallin-/- mice displayed a similar degree of stress tolerance showing that alphaB-crystallin is dispensable for protection of cells against energy depletion. The functional r...Continue Reading

Citations

Oct 1, 2005·Histochemistry and Cell Biology·Jens WaschkeRoger H Adamson
Jan 18, 2006·Histochemistry and Cell Biology·Jens WaschkeDetlev Drenckhahn
Oct 1, 2009·Physiological Reviews·Aikaterini Kontrogianni-KonstantopoulosRobert J Bloch
Nov 28, 2008·Virology Journal·Stephanie ErbarAndrea Maisner
Apr 19, 2005·Cell Stress & Chaperones·Pamela LouapreJean C David
Sep 7, 2012·Journal of Visualized Experiments : JoVE·Malgorzata BurekCarola Y Förster
Oct 23, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sergey ShityakovJens-Albert Broscheit
Mar 18, 2006·The Journal of Physiology·Carola FörsterDetlev Drenckhahn
Nov 11, 2008·Molecular and Cellular Endocrinology·Malgorzata Burek, Carola Y Förster
May 20, 2015·Biochimica Et Biophysica Acta·Vadde Sudhakar Reddy, G Bhanuprakash Reddy
May 6, 2006·Ultramicroscopy·F KienbergerP Hinterdorfer
Jun 21, 2014·Archives of Biochemistry and Biophysics·V Sudhakar ReddyG Bhanuprakash Reddy
Jan 10, 2008·International Immunopharmacology·Peter M BenzThomas Renné
Mar 25, 2014·Biochimica Et Biophysica Acta·Astrid SchrammelBernd Mayer
Mar 18, 2008·Journal of Molecular Biology·Werner BaumgartnerReinhard Gessner
Jan 24, 2009·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Yang LiuEn-Hua Wang
Jan 18, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Christina DillingMalgorzata Burek
Apr 30, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·M Rafiuddin AhmedR Jayakumar
Jul 24, 2004·American Journal of Physiology. Heart and Circulatory Physiology·J WaschkeF E Curry
May 11, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sharang GhavampourSüleyman Ergün
Nov 17, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Malgorzata BurekCarola Y Förster
Nov 6, 2004·American Journal of Physiology. Heart and Circulatory Physiology·J WaschkeD Drenckhahn
Nov 6, 2007·American Journal of Physiology. Cell Physiology·K Müller-MarschhausenD Drenckhahn
Nov 9, 2007·American Journal of Physiology. Cell Physiology·N SchlegelJ Waschke
Dec 17, 2002·Histochemistry and Cell Biology·Esther Asan
Sep 27, 2003·American Journal of Physiology. Heart and Circulatory Physiology·J WaschkeD Drenckhahn
Mar 11, 2016·The Journal of Toxicological Sciences·Sergey ShityakovCarola Förster
Nov 21, 2017·Cellular and Molecular Bioengineering·Mona AhmedBjörn Gustafsson
Jul 6, 2012·American Journal of Physiology. Cell Physiology·Noni T LarkinsGraham D Lamb
Oct 7, 2011·American Journal of Physiology. Cell Physiology·Noni T LarkinsGraham D Lamb
Mar 27, 2004·American Journal of Physiology. Heart and Circulatory Physiology·J WaschkeF E Curry
Feb 14, 2003·Journal of Cell Science·Werner BaumgartnerDetler Drenckhahn

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.