Expression and localisation of BDNF, NT4 and TrkB in proliferative vitreoretinopathy.

Experimental Eye Research
Seyed M S Ghazi-NouriDavid G Charteris

Abstract

Exogenous brain derived neurotrophic factor (BDNF) is known to rescue ganglion cell death after optic nerve injury. Its mechanism of action is believed to be indirect via glial cells in the retina. In this study we investigated the changes in expression and localisation of BDNF, neurotrophin-4 (NT4) and their common receptor (TrkB) in retinectomy sections of patients with proliferative vitreoretinopathy (PVR). Nine full-thickness retinectomy specimens obtained at retinal reattachment surgery for PVR were fixed in 4% paraformaldehyde immediately after excision and compared to similarly processed normal donor retinas (4 eyes). Agarose-embedded sections (100 microm thick) were double labelled for immunohistochemistry by confocal microscopy, with antibodies against BDNF, NT4, TrkB, rod opsin, glial fibrillary acidic protein (GFAP), cellular retinaldehyde binding protein (CRALBP) and Brn3. This study demonstrates expression of NT4 by ganglion cells and shows expression of BDNF and NT4 in the outer photoreceptor segments is downregulated during PVR, whilst NT4 is markedly upregulated throughout the retina during this condition. The findings here suggest that NT4 may play a neural protective role during the development of PVR. It also...Continue Reading

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Citations

Sep 22, 2009·American Journal of Human Genetics·Francesca PasuttoAndré Reis
Mar 15, 2018·The European Journal of Neuroscience·Rachael S AllenMachelle T Pardue
Aug 28, 2016·The British Journal of Ophthalmology·Petros PetrouDavid G Charteris
Feb 22, 2012·Melanoma Research·Tatyana MilmanPaul T Finger
Mar 16, 2019·BMC Medical Genomics·Darya V TeleginaOyuna S Kozhevnikova

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