Expression and localization of a novel Rab small G protein (Rab38) in the rat lung

The American Journal of Pathology
Kazuhiro OsanaiDennis R Voelker

Abstract

The Rab small G protein family participates in intracellular vesicle transport, including exocytosis and endocytosis. The cDNA encoding a novel Rab-related small G protein (Rab38) has been cloned from rat lung cDNA library and recorded in GenBank (accession no. M94043). However, the expression and localization of the protein in the lung remains primarily unknown. We produced polyhistidine-tagged recombinant Rab38 and a polyclonal antibody with a synthetic peptide. Immunohistochemistry demonstrated that the protein is specifically localized in alveolar type II cells and in bronchial epithelial cells. In situ hybridization using a digoxygenin-labeled RNA riboprobe clearly showed that the mRNA of the protein is localized in alveolar type II cells and bronchial epithelial cells, especially terminal airway epithelial cells. Western blot and reverse transcriptase-polymerase chain reaction showed distinct expression of the protein and mRNA in isolated alveolar type II cells, but not in alveolar macrophages. The native protein was predominantly hydrophobic and was enriched in a high-density vesicle fraction but was barely detectable in nuclear and lamellar body fractions in alveolar type II cells. Immunofluorescence cytochemistry perfo...Continue Reading

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Citations

May 24, 2006·Cancer Immunology, Immunotherapy : CII·Alfred ZippeliusDirk Jaeger
Mar 12, 2008·The Journal of Biological Chemistry·Deming GouLin Liu
Jul 19, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Linghui ZhangShaohui Huang
Apr 22, 2005·Biological Chemistry·Kazuhiro OsanaiDennis R Voelker
Nov 24, 2011·Nucleic Acids Research·Nina M MuñozHans-Peter Kiem
Oct 4, 2008·The American Journal of Pathology·Kazuhiro OsanaiDennis R Voelker
May 15, 2008·Biochemical and Biophysical Research Communications·Fang WangZhijie Chang
Nov 6, 2008·Journal of Thrombosis and Haemostasis : JTH·I NinkovicY H Datta
Nov 10, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kazuhiro OsanaiHirohisa Toga
Oct 18, 2006·The Journal of Cell Biology·Christina WasmeierMiguel C Seabra
Oct 18, 2006·The Journal of Cell Biology·Michael S Marks
Aug 1, 2018·International Journal of Molecular Sciences·Kazuhiro Osanai
Dec 21, 2018·Molecular Medicine Reports·Bao-Yu LiBin Liu
Mar 23, 2007·International Journal of Mass Spectrometry·Zhang-Zhi HuCathy Wu

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